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Literature summary for 1.13.11.2 extracted from

  • Hupert-Kocurek, K.; Stawicka, A.; Wojcieszynska, D.; Guzik, U.
    Cloning and mutagenesis of catechol 2,3-dioxygenase Gene from the Gram-positive Planococcus sp. strain S5 (2013), J. Mol. Microbiol. Biotechnol., 23, 381-390.
    View publication on PubMed
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
gene c23o, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes and recombinant expression in Escherichia coli strain BL21 Planococcus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
A23T/F212S random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
C103R/K289stop random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
F191I/C268R/Y272H/V280A/Y293D / random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
G127D/P140T random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
H24R/F168S/Q275R random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
L13R random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
M65T random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
R296Q random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
R296Q random mutagenesis, the mutant shows significant tolerance to acidic pH with an optimum at pH 5.0, as well as a over 1.5fold increased activity, altered substrate inhibition, and 2.5fold lower KM compared to the wild-type enzyme Planococcus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 catechol pH 5.0, 35°C, mutant R296Q Planococcus sp. (in: Bacteria)
0.04
-
 catechol pH 7.0, 35°C, mutant A23T/F212S Planococcus sp. (in: Bacteria)
0.053
-
 catechol pH 7.0, 35°C, mutant H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
0.09
-
 catechol pH 7.0, 35°C, wild-type enzyme Planococcus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Planococcus sp. (in: Bacteria)
-
 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2 Planococcus sp. (in: Bacteria) S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Planococcus sp. (in: Bacteria)
-
 gene c23o
-
Planococcus sp. (in: Bacteria) S5
-
 gene c23o
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00887
-
 pH 7.0, 35°C, mutant H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
0.01285
-
 pH 7.0, 35°C, mutant A23T/F212S Planococcus sp. (in: Bacteria)
0.01973
-
 pH 7.0, 35°C, wild-type enzyme Planococcus sp. (in: Bacteria)
0.03187
-
 pH 5.0, 35°C, mutant R296Q Planococcus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Planococcus sp. (in: Bacteria) 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2
-
 Planococcus sp. (in: Bacteria) S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
C23o
-
 Planococcus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 wild-type and mutant enzymes Planococcus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 mutant R296Q Planococcus sp. (in: Bacteria)
6.5
-
 mutants A23T/F212S and H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
7
-
 wild-type enzyme Planococcus sp. (in: Bacteria)

General Information

General Information Comment Organism
additional information the enzyme is a member of the superfamily I, subfamily 2A of extradiol dioxygenases Planococcus sp. (in: Bacteria)
physiological function catechol 2,3-dioxygenases play a role in the degradation of monoaromatic hydrocarbons Planococcus sp. (in: Bacteria)