1.1.1.76: (S,S)-butanediol dehydrogenase

This is an abbreviated version, for detailed information about (S,S)-butanediol dehydrogenase, go to the full flat file.

Reaction

(2S,3S)-butane-2,3-diol
+
NAD+
=
(S)-acetoin
+
NADH
+
H+

Synonyms

(2S, 3S)-BDH, (2S, 3S)-butanediol dehydrogenase, (S,S)-2,3-butanediol dehydrogenase, (S,S)-butanediol dehydrogenase, 2,3-BDH, 2,3-butanediol dehydrogenase, acetoin(diacetyl) reductase, AdR, ADS1, ARA1, BDH, budC, ButA, L(+)-2,3-butanediol dehydrogenase (L-acetoin forming), L-(2S,3S)-butanediol dehydrogenase, L-BDH, L-butanediol dehydrogenase, LBDH, meso-2,3-butanediol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.76 (S,S)-butanediol dehydrogenase

Cloned

Cloned on EC 1.1.1.76 - (S,S)-butanediol dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-expressed with glucose dehydrogenase and formate dehydrogenase in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
expression in Escherichia coli JM109, stereospecifity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecifity is exhibited; When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
-
expression in Escherichia coli JM109/pLBD2-CTC; L-butanediol gene containing plasmid pLBD2-CTC is expressed in Escherichia coli JM109
-
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)