BRENDA - Enzyme Database show
show all sequences of 1.1.1.76

Stereochemical applications of the expression of the L-2,3-butanediol dehydrogenase gene in Escherichia coli

Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Mimura, A.; Ohkuma, M.; Kudo, T.; Lett. Appl. Microbiol. 32, 93-98 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli JM109, stereospecifity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecifity is exhibited; When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
Corynebacterium glutamicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
-
C-1012
-
Corynebacterium glutamicum C-1012
-
C-1012
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid
Corynebacterium glutamicum
1.51
-
plasmid containing L-butanediol dehydrogenase gene
Corynebacterium glutamicum
17
-
plasmid containing tac promoter and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
18.6
-
plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum
(S,S)-butanediol + NAD+
-
-
-
?
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum C-1012
(S,S)-butanediol + NAD+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Corynebacterium glutamicum
NADH
-
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli JM109, stereospecifity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecifity is exhibited; When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
Corynebacterium glutamicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Corynebacterium glutamicum
NADH
-
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid
Corynebacterium glutamicum
1.51
-
plasmid containing L-butanediol dehydrogenase gene
Corynebacterium glutamicum
17
-
plasmid containing tac promoter and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
18.6
-
plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum
(S,S)-butanediol + NAD+
-
-
-
?
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum C-1012
(S,S)-butanediol + NAD+
-
-
-
?
Other publictions for EC 1.1.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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1
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1
1
1
-
-
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-
-
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1
1
1
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1
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2
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18
1
2
1
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-
2
1
-
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-
1
1
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737381
Shimegi
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Corynebacterium glutamicum
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1
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1
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1
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1
1
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1
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1
1
1
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1
1
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1
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1
1
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-
-
-
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-
737865
Takeda
Identification and characteriz ...
Mycobacterium sp., Mycobacterium sp. B-009
Biosci. Biotechnol. Biochem.
78
1879-1886
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-
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-
-
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1
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1
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3
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4
1
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1
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1
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1
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4
1
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1
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-
2
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-
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-
1
1
739652
Wang
Engineering of cofactor regene ...
Corynebacterium glutamicum
Sci. Rep.
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-
1
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1
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1
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1
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3
1
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1
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1
1
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1
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1
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3
1
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Saccharomyces cerevisiae
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-
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1
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1
1
-
-
-
712019
Otagiri
Structural basis for chiral su ...
Corynebacterium glutamicum
FEBS Lett.
584
219-223
2010
-
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-
1
3
-
1
-
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1
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2
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2
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1
3
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1
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2
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2
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389578
Ui
Stereochemical applications of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Lett. Appl. Microbiol.
32
93-98
2001
-
-
1
-
-
-
-
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-
-
-
-
-
9
-
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-
4
-
6
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2
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1
2
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4
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6
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389579
Otagiri
-
Crystallization and preliminar ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Protein Pept. Lett.
8
57-61
2001
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-
1
1
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6
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2
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1
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1
1
1
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2
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389580
Takusagawa
Purification and characterizat ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Biosci. Biotechnol. Biochem.
65
1876-1878
2001
1
1
1
-
-
-
9
5
-
6
3
-
-
8
-
-
1
-
-
-
2
-
12
1
-
-
2
-
2
-
1
2
1
1
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1
1
1
2
-
-
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9
1
5
-
6
3
-
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1
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2
-
12
1
-
-
2
-
2
-
1
1
-
-
-
-
-
-
389573
Ui
-
Mechanism for the formation of ...
Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063
J. Ferment. Technol.
62
551-559
1984
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3
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389574
Ui
Separation and qunatitation of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Agric. Biol. Chem.
48
2837-2838
1984
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1
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6
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1
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1
2
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2
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1
2
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2
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1
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389575
Ui
-
Laboratory-scale production of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012, Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063, no activity in Pseudomonas sp., no activity in Pseudomonas sp. s4, Paenibacillus polymyxa, Paenibacillus polymyxa IAM 1189, Saccharomyces cerevisiae, Saccharomyces cerevisiae OC-2, Serratia marcescens, Serratia marcescens IAM 1022
J. Ferment. Technol.
62
151-156
1984
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18
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2
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2
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389576
Voloch
Reduction of acetoin to 2,3-bu ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
25
173-183
1983
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1
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1
1
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2
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2
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1
1
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389577
Taylor
Stereoisomeric specificities o ...
Aeromonas hydrophila, Bacillus subtilis, Bacillus subtilis Ford, Enterobacter aerogenes, Paenibacillus polymyxa
Biochim. Biophys. Acta
39
448-457
1960
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5
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2
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