Application | Comment | Organism |
---|---|---|
synthesis | the key enzymes in the microbial production of 2,3-butanediol | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26864 | - |
2 * 26864, sequence calculation | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-butane-2,3-diol + NAD+ | Rhodococcus erythropolis | the enzyme displayed absolute stereospecificity in the reduction of diacetyl to (2S,3S)-2,3-butanediol via (S)-acetoin. Physiological role in favor of (2S,3S)-2,3-butanediol formation | (S)-acetoin + NADH + H+ | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | the enzyme exhibits remarkable tolerance to dimethyl sulfoxide and retains 53.6% of the initial activity after 4 h incubation with 30% v/v DMSO | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | M4N626 | - |
- |
Rhodococcus erythropolis WZ010 | M4N626 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
269 | - |
purified recombinant enzyme, (2S,3S)-2,3-butanediol oxidation, pH 9.5, 25°C | Rhodococcus erythropolis |
8519 | - |
purified recombinant enzyme, diacetyl reduction, pH 7.0, 30°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-butane-2,3-diol + NAD+ | the enzyme displayed absolute stereospecificity in the reduction of diacetyl to (2S,3S)-2,3-butanediol via (S)-acetoin. Physiological role in favor of (2S,3S)-2,3-butanediol formation | Rhodococcus erythropolis | (S)-acetoin + NADH + H+ | - |
r | |
(2S,3S)-butane-2,3-diol + NAD+ | the enzyme displays absolute stereospecificity in the reduction of diacetyl to (2S,3S)-2,3-butanediol via (S)-acetoin. Under the optimized conditions, the activity of diacetyl reduction is 11.9fold higher than that of (2S,3S)-2,3-butanediol oxidation | Rhodococcus erythropolis | (S)-acetoin + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | - |
Rhodococcus erythropolis | acetophenone + NADH + H+ | - |
r | |
1,2-propanediol + NAD+ | - |
Rhodococcus erythropolis | ? + NADH + H+ | - |
? | |
1-butanol + NAD+ | - |
Rhodococcus erythropolis | butanal + NADH + H+ | - |
? | |
1-butanol + NAD+ | - |
Rhodococcus erythropolis WZ010 | butanal + NADH + H+ | - |
? | |
1-phenylpropanol + NAD+ | - |
Rhodococcus erythropolis | 1-phenylpropan-1-one + NADH + H+ | - |
? | |
2,2,2-trifluoroacetophenone + NADH + H+ | - |
Rhodococcus erythropolis | ? + NAD+ | - |
? | |
2-butanol + NAD+ | - |
Rhodococcus erythropolis | 2-butanone + NADH + H+ | - |
? | |
2-butanol + NAD+ | - |
Rhodococcus erythropolis WZ010 | 2-butanone + NADH + H+ | - |
? | |
2-pentanol + NAD+ | - |
Rhodococcus erythropolis | 2-pentanone + NADH + H+ | - |
? | |
2-pentanol + NAD+ | - |
Rhodococcus erythropolis WZ010 | 2-pentanone + NADH + H+ | - |
? | |
cyclohexanol + NAD+ | - |
Rhodococcus erythropolis | cyclohexanone + NADH + H+ | - |
? | |
cyclohexanol + NAD+ | - |
Rhodococcus erythropolis WZ010 | cyclohexanone + NADH + H+ | - |
? | |
ethyl pyruvate + NADH + H+ | - |
Rhodococcus erythropolis | ? + NAD+ | - |
? | |
isopropanol + NAD+ | - |
Rhodococcus erythropolis | isopropanal + NADH + H+ | - |
? | |
additional information | the enzyme accepts a broad range of substrates including aliphatic and aryl alcohols, aldehydes, and ketones, overview. No activity with 4-chloroacetophenone, (R)-1-phenylethanol, and (2R,3R)-2,3-butanediol, poor activity with 3-methyl-2-acetophenone, 4-bromoacetophenone, 2-bromoacetophenone, benzaldehyde, and isophorone | Rhodococcus erythropolis | ? | - |
? | |
additional information | the enzyme accepts a broad range of substrates including aliphatic and aryl alcohols, aldehydes, and ketones, overview. No activity with 4-chloroacetophenone, (R)-1-phenylethanol, and (2R,3R)-2,3-butanediol, poor activity with 3-methyl-2-acetophenone, 4-bromoacetophenone, 2-bromoacetophenone, benzaldehyde, and isophorone | Rhodococcus erythropolis WZ010 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 26864, sequence calculation | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Rhodococcus erythropolis |
acetoin(diacetyl) reductase | - |
Rhodococcus erythropolis |
AdR | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
(2S,3S)-2,3-butanediol oxidation | Rhodococcus erythropolis |
30 | - |
diacetyl reduction | Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | activity range | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
diacetyl reduction | Rhodococcus erythropolis |
9.5 | - |
(2S,3S)-2,3-butanediol oxidation | Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 11 | activity range | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme binding motif T11G12XXXG16XG18 is conserved in the N-terminal region | Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family of the short-chain dehydrogenase/reductases | Rhodococcus erythropolis |