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1.1.1.76: (S,S)-butanediol dehydrogenase

This is an abbreviated version!
For detailed information about (S,S)-butanediol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.76

Reaction

(2S,3S)-butane-2,3-diol
+
NAD+
=
(S)-acetoin
+
NADH
+
H+

Synonyms

(2R,3R)-2,3-BDH, (2R,3R)-2,3-butanediol dehydrogenase, (2S, 3S)-BDH, (2S, 3S)-butanediol dehydrogenase, (S,S)-2,3-BDH, (S,S)-2,3-butanediol dehydrogenase, (S,S)-butanediol dehydrogenase, 2,3-BDH, 2,3-butanediol dehydrogenase, acetoin(diacetyl) reductase, AdR, ADS1, ARA1, BDH, budC, ButA, L(+)-2,3-butanediol dehydrogenase (L-acetoin forming), L-(2S,3S)-butanediol dehydrogenase, L-BDH, L-butanediol dehydrogenase, LBDH, mbdh, meso-2,3-butanediol dehydrogenase, More, S-2,3-butanediol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.76 (S,S)-butanediol dehydrogenase

Purification

Purification on EC 1.1.1.76 - (S,S)-butanediol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
-
HiTrap Q column chromatography and Superdex 200 gel filtration
-
protamine sufate precipitation
-
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant SUMO-His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-Gold by affinity chromatography, desalting gel filtration, and protease-mediated tag cleavage
streptomycin sulfate precipitation, affinity chromatography, gel filtration
-