Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.76 extracted from

  • Shimegi, T.; Ooyama, T.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.; Ui, S.
    Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase (2014), Acta Crystallogr. Sect. F, 70, 461-463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 17% (w/v) PEG 4000, 15% (v/v) glycerol and 1% (v/v) beta-mercaptoethanol in 100 mM MES buffer pH 6.4 Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
4 * 26000, SDS-PAGE Corynebacterium glutamicum
103000
-
gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S,3S)-butane-2,3-diol + NAD+ Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography and Superdex 200 gel filtration Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-butane-2,3-diol + NAD+
-
Corynebacterium glutamicum (2S)-acetoin + NADH + H+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 26000, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
L-(2S,3S)-butanediol dehydrogenase
-
Corynebacterium glutamicum
L-BDH
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Corynebacterium glutamicum