BRENDA - Enzyme Database show
show all sequences of 1.1.1.76

Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase

Shimegi, T.; Ooyama, T.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.; Ui, S.; Acta Crystallogr. Sect. F 70, 461-463 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Corynebacterium glutamicum
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 17% (w/v) PEG 4000, 15% (v/v) glycerol and 1% (v/v) beta-mercaptoethanol in 100 mM MES buffer pH 6.4
Corynebacterium glutamicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
103000
-
gel filtration
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3S)-butane-2,3-diol + NAD+
Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
-
-
-
Purification (Commentary)
Commentary
Organism
HiTrap Q column chromatography and Superdex 200 gel filtration
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-butane-2,3-diol + NAD+
-
737381
Corynebacterium glutamicum
(2S)-acetoin + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 26000, SDS-PAGE
Corynebacterium glutamicum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Corynebacterium glutamicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Corynebacterium glutamicum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 17% (w/v) PEG 4000, 15% (v/v) glycerol and 1% (v/v) beta-mercaptoethanol in 100 mM MES buffer pH 6.4
Corynebacterium glutamicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
103000
-
gel filtration
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3S)-butane-2,3-diol + NAD+
Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
HiTrap Q column chromatography and Superdex 200 gel filtration
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-butane-2,3-diol + NAD+
-
737381
Corynebacterium glutamicum
(2S)-acetoin + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 26000, SDS-PAGE
Corynebacterium glutamicum
Other publictions for EC 1.1.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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721396
Wang
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Rhodococcus erythropolis, Rhodococcus erythropolis WZ010
Appl. Microbiol. Biotechnol.
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1
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1
1
1
5
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1
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2
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18
1
2
1
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2
1
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1
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-
1
1
1
-
-
-
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-
-
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1
1
1
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1
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2
-
18
1
2
1
-
-
2
1
-
-
-
1
1
-
-
-
737381
Shimegi
Crystallization and preliminar ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
70
461-463
2014
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1
1
-
-
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-
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1
1
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1
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1
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1
1
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1
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1
1
1
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1
1
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1
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1
1
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737865
Takeda
Identification and characteriz ...
Mycobacterium sp., Mycobacterium sp. B-009
Biosci. Biotechnol. Biochem.
78
1879-1886
2014
-
-
-
-
-
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-
1
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1
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3
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4
1
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1
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1
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1
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4
1
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1
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2
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1
1
739652
Wang
Engineering of cofactor regene ...
Corynebacterium glutamicum
Sci. Rep.
3
2643
2013
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-
1
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1
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1
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1
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3
1
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1
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1
1
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1
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1
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3
1
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710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
-
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1
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1
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1
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1
1
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-
712019
Otagiri
Structural basis for chiral su ...
Corynebacterium glutamicum
FEBS Lett.
584
219-223
2010
-
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-
1
3
-
1
-
-
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1
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2
-
2
-
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1
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1
3
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1
1
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2
-
2
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389578
Ui
Stereochemical applications of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Lett. Appl. Microbiol.
32
93-98
2001
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1
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9
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4
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6
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2
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1
2
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4
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6
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389579
Otagiri
-
Crystallization and preliminar ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Protein Pept. Lett.
8
57-61
2001
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-
1
1
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6
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2
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1
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1
1
1
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2
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389580
Takusagawa
Purification and characterizat ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Biosci. Biotechnol. Biochem.
65
1876-1878
2001
1
1
1
-
-
-
9
5
-
6
3
-
-
8
-
-
1
-
-
-
2
-
12
1
-
-
2
-
2
-
1
2
1
1
-
1
1
1
2
-
-
-
-
9
1
5
-
6
3
-
-
-
-
1
-
-
2
-
12
1
-
-
2
-
2
-
1
1
-
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-
-
-
-
389573
Ui
-
Mechanism for the formation of ...
Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063
J. Ferment. Technol.
62
551-559
1984
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3
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4
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1
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4
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1
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389574
Ui
Separation and qunatitation of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Agric. Biol. Chem.
48
2837-2838
1984
-
-
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1
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6
-
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1
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1
1
2
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2
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1
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2
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2
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1
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1
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1
1
2
-
2
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1
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389575
Ui
-
Laboratory-scale production of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012, Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063, no activity in Pseudomonas sp., no activity in Pseudomonas sp. s4, Paenibacillus polymyxa, Paenibacillus polymyxa IAM 1189, Saccharomyces cerevisiae, Saccharomyces cerevisiae OC-2, Serratia marcescens, Serratia marcescens IAM 1022
J. Ferment. Technol.
62
151-156
1984
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1
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18
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2
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4
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1
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2
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4
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4
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1
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2
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4
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1
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2
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389576
Voloch
Reduction of acetoin to 2,3-bu ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
25
173-183
1983
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1
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1
1
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2
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2
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1
1
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389577
Taylor
Stereoisomeric specificities o ...
Aeromonas hydrophila, Bacillus subtilis, Bacillus subtilis Ford, Enterobacter aerogenes, Paenibacillus polymyxa
Biochim. Biophys. Acta
39
448-457
1960
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