1.1.1.53: 3alpha(or 20beta)-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 3alpha(or 20beta)-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

androstan-3alpha,17beta-diol
+
NAD+
=
17beta-hydroxyandrostan-3-one
+
NADH
+
H+

Synonyms

(R)-20-hydroxysteroid dehydrogenase, 20-hydroxysteroid dehydrogenase, 20beta-HSD, 20beta-HSD type 2, 20beta-hydroxysteroid dehydrogenase, 20HSD, 3-alpha hydroxysteroid dehydrogenase type 3, 3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, carbonyl reductase-like 20beta-HSD, carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase, carbonyl reductase/20beta-hydroxysteroid dehydrogenase A, carbonyl reductase/20beta-hydroxysteroid dehydrogenase B, cortisone reductase, CR/20beta-HSD, CR/20beta-HSD A, CR/20beta-HSD B, dehydrogenase, 20beta-hydroxy steroid, delta4-3-ketosteroid hydrogenase, NADH-20beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.53 3alpha(or 20beta)-hydroxysteroid dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.53 - 3alpha(or 20beta)-hydroxysteroid dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
purified enzyme, NADPH, progesterone
0.004
-
-
0.019
-
purified enzyme, NADPH, 5beta-androstan-3alpha,17beta-diol oxidation
0.023
-
purified enzyme, NADPH, dehydroisoandrosterone
0.025
-
purified enzyme, NADPH, 5alpha-androstan-3alpha-ol-17-one oxidation
0.038
-
NADPH, phenylglyoxal
0.039
-
NADPH, 5alpha-dihydrotestosterone; NADPH, 5beta-dihydrotestosterone
0.042
-
NADPH, cyclohexanone
0.043
-
NADPH, 4-nitrobenzaldehyde
0.045
-
NADPH, camphorquinone
0.046
-
purified enzyme, NADPH, 5alpha-androstan-17beta-ol-3-one
0.051
-
purified enzyme, NADPH, 5alpha-pregnane-3,20-dione
0.052
-
purified enzyme, NADPH, 5alpha-androstan-3alpha,17beta-diol oxidation
0.091
-
purified enzyme, NADPH, 17alpha-methyl-5alpha-androstan-17beta-ol-3-one
0.123
-
NADPH, methylglyoxal
0.157
-
NADPH, phenanthrenequinone
0.167
-
NADPH, hydrindantin
0.27
-
enzyme type B mutant Q27K, 17alpha-hydroxyprogesterone
1.57
-
enzyme type A, 17alpha-hydroxyprogesterone
1.64
-
enzyme type B mutant T15I, 17alpha-hydroxyprogesterone
additional information
-
specific activity about 3.8 pmol/min/mg in ovulated oocyte microsomes and 6.9 pmol/min/mg in ovulated oocyte mitochondria, 11.2 pmol/min/mg in ovarian tissue mitochondria after ovulation