1.1.1.53: 3alpha(or 20beta)-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 3alpha(or 20beta)-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

androstan-3alpha,17beta-diol
+
NAD+
=
17beta-hydroxyandrostan-3-one
+
NADH
+
H+

Synonyms

(R)-20-hydroxysteroid dehydrogenase, 20-hydroxysteroid dehydrogenase, 20beta-HSD, 20beta-HSD type 2, 20beta-hydroxysteroid dehydrogenase, 20HSD, 3-alpha hydroxysteroid dehydrogenase type 3, 3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, carbonyl reductase-like 20beta-HSD, carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase, carbonyl reductase/20beta-hydroxysteroid dehydrogenase A, carbonyl reductase/20beta-hydroxysteroid dehydrogenase B, cortisone reductase, CR/20beta-HSD, CR/20beta-HSD A, CR/20beta-HSD B, dehydrogenase, 20beta-hydroxy steroid, delta4-3-ketosteroid hydrogenase, NADH-20beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.53 3alpha(or 20beta)-hydroxysteroid dehydrogenase

KM Value

KM Value on EC 1.1.1.53 - 3alpha(or 20beta)-hydroxysteroid dehydrogenase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.19
11alpha-Hemisuccinyloxyprogesterone
-
-
0.49
17-Hydroxy-3,11,20-trioxo-4-pregnen-21-oate
-
-
0.64
17-Hydroxy-3,20-dioxo-4-pregnen-21-oate
-
-
0.0105
17-hydroxyprogesterone
-
-
0.00094
17alpha-hydroxyprogesterone
-
reduction
0.46
17beta-hydroxyandrostan-2-one
-
-
0.017
20beta-hydroxyprogesterone
pH 6.0, 30C, reaction with NAD+, mutant enzyme I6T/V47M/T69K
2.23
21-Acetoxyprogesterone
-
-
1.43
21-Hemisuccinyloxyprogesterone
-
-
0.0251
21-hydroxyprogesterone
-
-
0.14
3,20-Dioxo-4-pregnen-21-oic acid
0.00018
5alpha-androstan-17beta-ol-3-one
-
reduction
0.00032
5alpha-Androstan-3alpha-ol-17-one
-
oxidation
0.00218 - 0.07103
5alpha-dihydrotestosterone
0.00016 - 0.0045
5alpha-pregnane-3,20-dione
0.02
6beta-[bromoacetoxy]-progesterone
-
-
126.1
alpha-NADH
-
-
328
alpha-NADPH
-
-
0.024
androsterone
pH 6.0, 30C, reaction with NAD+, mutant enzyme I6T/V47M/T69K
88.2
beta-NAD+
-
-
294.8
beta-NADH
-
-
14.8
beta-NADP+
-
-
7.2
beta-NADPH
-
-
0.024 - 0.318
cortisone
0.0086
deoxycorticosterone
-
-
0.0035 - 0.029
NADH
0.004
pregnonolone
-
-
-
0.00015 - 0.0061
progesterone
additional information
additional information
-
-
-