1.1.1.53: 3alpha(or 20beta)-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 3alpha(or 20beta)-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

androstan-3alpha,17beta-diol
+
NAD+
=
17beta-hydroxyandrostan-3-one
+
NADH
+
H+

Synonyms

(R)-20-hydroxysteroid dehydrogenase, 20-hydroxysteroid dehydrogenase, 20beta-HSD, 20beta-HSD type 2, 20beta-hydroxysteroid dehydrogenase, 20HSD, 3-alpha hydroxysteroid dehydrogenase type 3, 3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, carbonyl reductase-like 20beta-HSD, carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase, carbonyl reductase/20beta-hydroxysteroid dehydrogenase A, carbonyl reductase/20beta-hydroxysteroid dehydrogenase B, cortisone reductase, CR/20beta-HSD, CR/20beta-HSD A, CR/20beta-HSD B, dehydrogenase, 20beta-hydroxy steroid, delta4-3-ketosteroid hydrogenase, NADH-20beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.53 3alpha(or 20beta)-hydroxysteroid dehydrogenase

Engineering

Engineering on EC 1.1.1.53 - 3alpha(or 20beta)-hydroxysteroid dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
V54L
-
the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme
I6T/V47M/T69K
mutant enzyme with a maximal improvement in solubility, high catalytic activity as a NADP-dependent 3alpha,20beta-hydroxysteroid dehydrogenase
I6T/V47M/T69K/E142A
pH optimum for reduction of progesterone shifts slightly from pH 6.0 to pH 6.5 compared to for mutant enzyme I6T/V47M/T69K, the oxidation of androsterone shows a dual pH optimum, one at pH 6.0-6.5 and the other at pH 10
I6T/V47M/T69K/S140A
both oxidation of androsterone and reduction of progesterone are completely lost
I6T/V47M/T69K/Y153F
both oxidation of androsterone and reduction of progesterone are completely lost
I15T
-
in 20beta-HSD type A, cofactor binding is abolished
Q27K
-
in 20beta-HSD type B, cofactor binding is reduced
T15I
-
in 20beta-HSD type B, enzyme activity is restored