tropinone reductase II

This is an abbreviated version, for detailed information about tropinone reductase II, go to the full flat file.




DnTR2, More, pseudotropine forming tropinone reductase, pseudotropine-forming tropinone reductase, pseudotropinone forming tropinone reductase, TR-II, TR2, TRII, tropinone (psi-tropine-forming) reductase, tropinone reductase, tropinone reductase I, tropinone reductase II, tropinone reductase-II


     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
       tropinone reductase II


Cloned on EC - tropinone reductase II

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cDNA coding for TRI and TRII isolated and expressed in Escherichia coli NM522, P29X-encoding gene detected
cDNA encoding tropinone reductase II
cDNA homologous to pseudotropine-forming tropinone reductase isolated, recombinant protein functionally expressed in Escherichia coli BL21(DE3), transformed with pET21d vectors
expressed in Escherichia coli
full-length sequences determined of 5 clones with high homology to TRs
gene tr, DNA and amino acid sequence determination and analysis, sequence comparison
gene TR2, enzyme overexpression via leaves transformation with Agrobacterium tumefaciens AGL0, quantitative PCR enzyme expression analysis
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview
gene TRII, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), realtime PCR enzyme expression analysis
overexpression in Atropa belladonna. Transformation with cDNA of tropinone reductases successfully alters the ratio of tropine-derived alkaloids versus pseudotropine-derived alkaloids
P29X-encoding gene detected
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3)
TRII cDNA, in Escherichia coli expression vector pET21d