1.1.1.236: tropinone reductase II

This is an abbreviated version, for detailed information about tropinone reductase II, go to the full flat file.

Reaction

pseudotropine
+
NADP+
=
tropinone
+
NADPH
+
H+

Synonyms

More, pseudotropine forming tropinone reductase, pseudotropine-forming tropinone reductase, pseudotropinone forming tropinone reductase, TR-II, TRII, tropinone (psi-tropine-forming) reductase, tropinone reductase, tropinone reductase I, tropinone reductase II, tropinone reductase-II

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.236 tropinone reductase II

Cloned

Cloned on EC 1.1.1.236 - tropinone reductase II

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA coding for TRI and TRII isolated and expressed in Escherichia coli NM522, P29X-encoding gene detected
-
cDNA encoding tropinone reductase II
-
cDNA homologous to pseudotropine-forming tropinone reductase isolated, recombinant protein functionally expressed in Escherichia coli BL21(DE3), transformed with pET21d vectors
-
expressed in Escherichia coli
full-length sequences determined of 5 clones with high homology to TRs
-
gene tr, DNA and amino acid sequence determination and analysis, sequence comparison
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview
-
overexpression in Atropa belladonna. Transformation with cDNA of tropinone reductases successfully alters the ratio of tropine-derived alkaloids versus pseudotropine-derived alkaloids
-
P29X-encoding gene detected
-
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3)
-
TRII cDNA, in Escherichia coli expression vector pET21d