Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.236: tropinone reductase II

This is an abbreviated version!
For detailed information about tropinone reductase II, go to the full flat file.

Word Map on EC 1.1.1.236

Reaction

pseudotropine
+
NADP+
=
tropinone
+
NADPH
+
H+

Synonyms

DnTR2, More, pseudotropine forming tropinone reductase, pseudotropine reductase II, pseudotropine-forming tropinone reductase, pseudotropinone forming tropinone reductase, TR-II, TR2, TRII, tropinone (psi-tropine-forming) reductase, tropinone reductase, tropinone reductase I, tropinone reductase II, tropinone reductase-II

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.236 tropinone reductase II

Engineering

Engineering on EC 1.1.1.236 - tropinone reductase II

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y201V
site-directed mutagenesis, the mutant enzyme shows restored activity with tropinone, while the wild-type is inactive with tropinone. The mutant enzymes also remain to have the ability to reduce the 3-quinuclidinone and 4-methylcyclohexanone, although the Vmax of DnTR2-Y201V toward 4-methylcyclohexanone and 3-quinuclidinone are significantly lower (23 and 3fold decrease, respectively) than that of wild-type DnTR2 protein
additional information
-
enzyme suppression in potato plants by RNA interference (RNAi) and enzyme overexpression. The overexpression and gene knockdown of StTRII alters tropinone reduction in vivo, and has great influence on calystegine accumulation, in contrast to genetic manipulation of isozyme St-TRI, EC 1.1.1.206, phenotypes, calystegine analysis, overview. A strong reduction of calystegines appears in the StTRII RNAi lines