BRENDA - Enzyme Database show
show all sequences of 1.1.1.236

Protein structure modeling indicates hexahistidine-tag interference with enzyme activity

Freydank, A.C.; Brandt, W.; Draeger, B.; Proteins 72, 173-183 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview
Solanum dulcamara
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the recombinant His-tagged enzyme shows an increased substrate affinity compared to the wild-type enzyme
Solanum dulcamara
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant His-tagged enzyme, SDS-PAGE
Solanum dulcamara
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Solanum dulcamara
the enzyme is involved in tropane alkaloid biosynthesis, overview
pseudotropine + NADP+
leading to formation of calystegines
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum dulcamara
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Solanum dulcamara
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with quinuclidione and 8-thiabicyclo[3.2.1]octan-2-one
689953
Solanum dulcamara
?
-
-
-
-
tropinone + NADPH + H+
the enzyme is involved in tropane alkaloid biosynthesis, overview
689953
Solanum dulcamara
pseudotropine + NADP+
leading to formation of calystegines
-
-
?
tropinone + NADPH + H+
active site structure and catalytic triad, the recombinant His-tagged enzyme shows 4fold higher activity compared to the wild-type enzyme, overview
689953
Solanum dulcamara
pseudotropine + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 29000, recombinant His-tagged enzyme, SDS-PAGE
Solanum dulcamara
More
structure modelling, overview
Solanum dulcamara
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum dulcamara
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
recombinant His-tagged enzyme
Solanum dulcamara
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
the recombinant His-tagged enzyme shows a broader pH spectrum compared to the wild-type enzyme
Solanum dulcamara
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
binding site sequence, overview
Solanum dulcamara
Cloned(Commentary) (protein specific)
Commentary
Organism
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview
Solanum dulcamara
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
binding site sequence, overview
Solanum dulcamara
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the recombinant His-tagged enzyme shows an increased substrate affinity compared to the wild-type enzyme
Solanum dulcamara
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant His-tagged enzyme, SDS-PAGE
Solanum dulcamara
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Solanum dulcamara
the enzyme is involved in tropane alkaloid biosynthesis, overview
pseudotropine + NADP+
leading to formation of calystegines
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Solanum dulcamara
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with quinuclidione and 8-thiabicyclo[3.2.1]octan-2-one
689953
Solanum dulcamara
?
-
-
-
-
tropinone + NADPH + H+
the enzyme is involved in tropane alkaloid biosynthesis, overview
689953
Solanum dulcamara
pseudotropine + NADP+
leading to formation of calystegines
-
-
?
tropinone + NADPH + H+
active site structure and catalytic triad, the recombinant His-tagged enzyme shows 4fold higher activity compared to the wild-type enzyme, overview
689953
Solanum dulcamara
pseudotropine + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29000, recombinant His-tagged enzyme, SDS-PAGE
Solanum dulcamara
More
structure modelling, overview
Solanum dulcamara
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum dulcamara
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
recombinant His-tagged enzyme
Solanum dulcamara
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
the recombinant His-tagged enzyme shows a broader pH spectrum compared to the wild-type enzyme
Solanum dulcamara
Other publictions for EC 1.1.1.236
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726209
Dehghan
An atypical pattern of accumul ...
Hyoscyamus muticus, Hyoscyamus senecionis
Plant Physiol. Biochem.
70C
188-194
2013
-
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-
-
-
-
-
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-
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2
-
2
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2
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2
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2
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3
-
-
2
-
-
-
-
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-
-
-
-
-
2
2
-
-
-
696874
Kai
Molecular cloning, characteriz ...
Anisodus acutangulus
Biotechnol. Appl. Biochem.
54
:177-186
2009
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
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3
-
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1
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1
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1
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1
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3
-
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1
-
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-
689548
Brock
The functional divergence of s ...
Cochlearia officinalis
Plant J.
54
388-401
2008
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
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4
1
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1
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2
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4
1
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-
-
-
-
-
-
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-
689953
Freydank
Protein structure modeling ind ...
Solanum dulcamara
Proteins
72
173-183
2008
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
2
1
-
-
-
1
1
-
1
-
-
-
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-
1
1
-
-
-
-
-
-
1
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-
1
1
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-
-
1
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-
-
-
3
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
670518
Draeger
Tropinone reductases, enzymes ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, Solanum tuberosum
Phytochemistry
67
327-337
2006
-
-
4
-
-
-
-
18
-
-
4
-
-
4
-
-
-
-
-
4
4
-
14
-
-
-
-
1
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
-
-
18
-
-
4
-
-
-
-
-
-
4
4
-
14
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
670636
Kaiser
Immunolocalisation of two trop ...
Solanum tuberosum
Planta
225
127-137
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
4
-
-
1
-
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-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656413
Richter
Overexpression of tropinone re ...
Datura stramonium
J. Exp. Bot.
56
645-652
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
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-
-
-
-
-
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-
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2
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-
1
2
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654707
Yamashita
Capturing enzyme structure pri ...
Datura stramonium
Biochemistry
42
5566-5573
2003
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
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1
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2
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1
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1
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2
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-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
348033
Keiner
Molecular cloning, expression ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, Solanum tuberosum
Plant Mol. Biol.
48
299-308
2002
-
-
2
1
-
-
-
4
-
-
-
4
-
7
-
-
2
-
-
17
-
-
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
1
-
-
-
-
-
4
-
-
-
4
-
-
-
2
-
17
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347972
Nakajima
Site-directed mutagenesis of p ...
Arabidopsis sp., Datura stramonium, Hyoscyamus niger
J. Biol. Chem.
274
16563-16568
1999
-
-
3
1
-
-
-
19
-
-
-
2
-
3
-
-
2
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
19
-
-
-
2
-
-
-
2
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347973
Boswell
Specificities of the enzymes o ...
Brugmansia x candida, Datura stramonium, Datura stramonium D15/5, Hyoscyamus niger
Phytochemistry
52
871-878
1999
-
-
-
-
-
-
-
6
-
-
-
3
-
9
-
-
2
-
-
3
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
6
-
-
-
3
-
-
-
2
-
3
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348030
Nakajima
Two tropinone reductases, that ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, no activity in Nicotiana tabacum
Plant Cell Physiol.
40
1099-1107
1999
-
-
2
1
-
-
-
-
-
-
1
3
-
4
-
-
2
-
-
4
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
3
-
-
-
2
-
4
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348032
Yamashita
Structure of tropinone reducta ...
Datura stramonium
Biochemistry
38
7630-7637
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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2
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1
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2
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-
-
-
-
-
-
-
-
-
-
-
348028
Nakajima
Crystal structures of two trop ...
Datura stramonium, Hyoscyamus niger
Proc. Natl. Acad. Sci. USA
95
4876-4881
1998
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
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-
-
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2
2
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1
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1
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2
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-
-
2
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
348027
Portsteffen
The reduction of tropinone in ...
Atropa belladonna, Datura stramonium
Phytochemistry
37
391-400
1994
-
-
1
-
-
-
-
8
-
-
5
2
-
3
-
-
2
-
-
2
1
-
8
2
-
-
-
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
-
-
5
2
-
-
-
2
-
2
1
-
8
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
348026
Nakajima
Two tropinone reductases with ...
Atropa belladonna, Datura stramonium, Hyoscyamus niger, no activity in Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
90
9591-9595
1993
-
-
2
-
-
-
-
-
-
-
-
3
-
8
-
-
1
-
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3
-
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3
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2
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3
-
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1
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3
-
-
3
-
-
-
-
-
-
-
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-
-
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-
-
-
-
348023
Draeger
Levels of tropinone reductase ...
Atropa belladonna, Datura inoxia, Datura stramonium, Hyoscyamus niger, Physalis peruviana
Planta
188
581-586
1992
-
-
-
-
-
-
-
1
-
-
-
5
-
6
-
-
2
-
-
4
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
-
-
2
-
4
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348024
Hashimoto
Two tropinone reductases with ...
Hyoscyamus niger
Plant Physiol.
100
836-845
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348025
Portsteffen
-
Two tropinone reducing enzymes ...
Atropa belladonna, Calystegia sepium, Datura inoxia, Datura stramonium, Hyoscyamus bohemicus, Hyoscyamus canariensis, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus pusillus, no activity in Brassica campestris, no activity in Browallia americana, no activity in Nicotiana tabacum, Physalis alkekengi, Physalis edulis, Physalis philadelphica, Physochlaina orientalis
Phytochemistry
31
1135-1138
1992
-
-
-
-
-
3
1
16
-
-
4
13
-
16
-
-
2
-
-
12
1
1
30
4
-
-
-
-
2
2
1
2
-
-
-
-
-
-
2
-
-
3
-
1
-
16
-
-
4
13
-
-
-
2
-
12
1
1
30
4
-
-
-
-
2
2
1
-
-
-
-
-
-
-
348022
Couladis
-
Enzymes catalysing the reducti ...
Atropa belladonna, Datura inoxia, Datura stramonium, Hyoscyamus niger
Phytochemistry
30
801-805
1991
-
-
-
-
-
2
-
-
-
-
-
4
-
4
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2
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4
1
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14
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1
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-
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4
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6
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6
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2
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4
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-
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2
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4
1
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14
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1
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4
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348021
Draeger
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Purification and characterizat ...
Datura stramonium, Hyoscyamus niger
Agric. Biol. Chem.
52
2663-2667
1988
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1
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15
2
1
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1
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1
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1
4
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4
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4
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2
2
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1
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2
1
2
15
2
1
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1
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1
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1
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