1.1.1.18: inositol 2-dehydrogenase

This is an abbreviated version, for detailed information about inositol 2-dehydrogenase, go to the full flat file.

Reaction

myo-inositol
+
NAD+
=
2,4,6/3,5-pentahydroxycyclohexanone
+
NADH
+
H+

Synonyms

BsIDH, IDH, inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase, inositol dehydrogenase, iolG, iolG1, iolG2, LcIDH1, LcIDH2, MI dehydrogenase, More, myo-inositol 2-dehydrogenase, myo-inositol dehydrogenase, myo-inositol:NAD2 oxidoreductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.18 inositol 2-dehydrogenase

Engineering

Engineering on EC 1.1.1.18 - inositol 2-dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A12K/D35S/V36R
-
site-directed mutagenesis, the triple mutant has a value of 570000 M/s in reaction with NADP+, higher than that of the wild-type IDH with NAD+. The binding of the coenzyme in the mutant is altered such that although the nicotinamide ring maintains the required position for catalysis, the coenzyme has twisted by nearly 90°, so the adenine moiety no longer binds to a hydrophobic cleft in the Rossmann fold as in the wild-type enzyme
D172N
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D35S/V36R
-
site-directed mutagenesis, the double mutant prefers NADP+ to NAD+ by a factor of 5. The mutant is an excellent catalyst with a second-order rate constant with respect to NADP of 370000 M/s
H176A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
K97V
-
site-directed mutagenesis, inactive mutant
up
-
iolG expression is induced by myo-inositol, and less by scyllo-inositol
Y233F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y233R
-
site-directed mutagenesis, inactive mutant
Y235F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y235R
-
site-directed mutagenesis, inactive mutant
up
-
iolG expression is induced by myo-inositol, and less by scyllo-inositol
-
analysis
-
specific determination of myo-inositol using a fluorophotometer to measure the fluorescence of NADH released by enzyme immobilized on porous glass
additional information
-
convertion of NAD+-specific inositol dehydrogenase to an efficient NADP+-selective catalyst to enhance understanding of coenzyme selectivity and to create an enzyme capable of recycling NADP+ in biocatalytic processes