1.1.1.18: inositol 2-dehydrogenase

This is an abbreviated version, for detailed information about inositol 2-dehydrogenase, go to the full flat file.

Reaction

myo-inositol
+
NAD+
=
2,4,6/3,5-pentahydroxycyclohexanone
+
NADH
+
H+

Synonyms

BsIDH, IDH, inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase, inositol dehydrogenase, iolG, MI dehydrogenase, More, myo-inositol 2-dehydrogenase, myo-inositol dehydrogenase, myo-inositol:NAD2 oxidoreductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.18 inositol 2-dehydrogenase

Engineering

Engineering on EC 1.1.1.18 - inositol 2-dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D172N
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
H176A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
K97V
-
site-directed mutagenesis, inactive mutant
up
-
iolG expression is induced by myo-inositol, and less by scyllo-inositol
Y233F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y233R
-
site-directed mutagenesis, inactive mutant
Y235F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y235R
-
site-directed mutagenesis, inactive mutant
up
-
iolG expression is induced by myo-inositol, and less by scyllo-inositol
-
analysis
-
specific determination of myo-inositol using a fluorophotometer to measure the fluorescence of NADH released by enzyme immobilized on porous glass