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Literature summary for 1.1.1.18 extracted from

  • Daniellou, R.; Zheng, H.; Langill, D.M.; Sanders, D.A.; Palmer, D.R.
    Probing the promiscuous active site of myo-inositol dehydrogenase using synthetic substrates, homology modeling, and active site modification (2007), Biochemistry, 46, 7469-7477.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D172N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Bacillus subtilis
H176A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Bacillus subtilis
Y233F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Bacillus subtilis
Y233R site-directed mutagenesis, inactive mutant Bacillus subtilis
Y235F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Bacillus subtilis
Y235R site-directed mutagenesis, inactive mutant Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate inactivation Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NAD+ pH 9.0, 25°C, recombinant mutant Y233F Bacillus subtilis
0.08
-
NAD+ pH 9.0, 25°C, recombinant wild-type enzyme Bacillus subtilis
0.11
-
NAD+ pH 9.0, 25°C, recombinant mutant Y235F Bacillus subtilis
0.3
-
NAD+ pH 9.0, 25°C, recombinant mutant H176A Bacillus subtilis
0.4
-
NAD+ pH 9.0, 25°C, recombinant mutant D179N Bacillus subtilis
1.1
-
NAD+ pH 9.0, 25°C, recombinant mutant D172N Bacillus subtilis
4
-
myo-inositol pH 9.0, 25°C, recombinant mutant Y233F Bacillus subtilis
4.4
-
myo-inositol pH 9.0, 25°C, recombinant wild-type enzyme Bacillus subtilis
28
-
myo-inositol pH 9.0, 25°C, recombinant mutant D179N Bacillus subtilis
39
-
myo-inositol pH 9.0, 25°C, recombinant mutant Y235F Bacillus subtilis
65
-
myo-inositol pH 9.0, 25°C, recombinant mutant D172N Bacillus subtilis
118
-
myo-inositol pH 9.0, 25°C, recombinant mutant H176A Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + NAD+ Bacillus subtilis
-
scyllo-inosose + NADH
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P26935
-
-

Reaction

Reaction Comment Organism Reaction ID
myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ ordered sequential Bi-Bi mechanism in the absence of products, residues Y233, Y235, H176, and D172 are important for activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylbenzenesulfonyl-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-((1S)-10-camphor-sulfonyl)-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-((4-methyloxycarbonyl)-benzyl)-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-(4-carboxybenzyl)-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-(trans-cinnamoyl)-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-allyl-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-alpha-D-glucopyranosyl-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-benzyl-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-methyl-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-[(2-methylphenyl)methyl]-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
4-O-[(3-methylphenyl)methyl]-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?
additional information substrate specificity and substrate binding structure, molecular modeling, overview Bacillus subtilis ?
-
?
myo-inositol + NAD+
-
Bacillus subtilis scyllo-inosose + NADH
-
r
myo-inositol + NAD+
-
Bacillus subtilis scyllo-inosose + NADH + H+
-
?
[(4-methylphenyl)methyl]-myo-inositol + NAD+
-
Bacillus subtilis ? + NADH + H+
-
?

Subunits

Subunits Comment Organism
More construction of an homology model of inositol dehydrogenase, to which NADH and 4-O-benzylscyllo-inosose are docked and the active site energy minimized, molecular modeling, overview Bacillus subtilis

Synonyms

Synonyms Comment Organism
IDH
-
Bacillus subtilis
myo-inositol dehydrogenase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
myo-inositol pH 9.0, 25°C, recombinant mutant H176A Bacillus subtilis
1.9
-
myo-inositol pH 9.0, 25°C, recombinant mutant D172N Bacillus subtilis
34
-
myo-inositol pH 9.0, 25°C, recombinant mutant Y235F Bacillus subtilis
47
-
myo-inositol pH 9.0, 25°C, recombinant mutant Y233F Bacillus subtilis
58
-
myo-inositol pH 9.0, 25°C, recombinant wild-type enzyme Bacillus subtilis
73
-
myo-inositol pH 9.0, 25°C, recombinant mutant D179N Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis