EC Number   |
Protein Variants |
Reference |
|---|
   7.4.2.5 | E599Q |
3000fold reduction of ATPase activity compared to wild-type |
656145 |
| 7.4.2.5 | more |
a collection of 63 monocysteine mutants for the 901-aminoacid-residue SecA protein is generated for topological analysis of the protein |
687665 |
| 7.4.2.5 | D209A |
a dominant-negative mutant, binds ATP but is unable to hydrolyze it and is inactive in proOmpA translocation. Mutant generates a translocation intermediate of 18 kDa. Further addition of wild-type SecA causes its translocation into either mature OmpA or another intermediate of 28 kDa that can be translocated into mature by a proton motive force. The addition of excess D209N SecA during translocation causes a topology inversion of SecG |
720044 |
| 7.4.2.5 | more |
analysis of the function of SecA via mutants and SecA-SecA2-chimeras |
698615 |
| 7.4.2.5 | more |
analysis of the function of SecA1 and SecA2 via several mutants |
699002 |
| 7.4.2.5 | more |
analysis of the function of SecA2 via mutants and SecA-SecA2-chimeras |
698615 |
| 7.4.2.5 | more |
analysis of the function of the two-helix finger of the SecA ATPase via point mutations |
700390 |
| 7.4.2.5 | D630M |
ATPase activity is 0.6% of wild-type activity |
-, 667495 |
| 7.4.2.5 | K508M |
ATPase activity is 1.3% of wild-type activity |
-, 667495 |
| 7.4.2.5 | F197I |
change in selectivity |
719940 |
