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Literature summary for 7.4.2.5 extracted from

  • Bensing, B.A.; Sullam, P.M.
    Characterization of Streptococcus gordonii SecA2 as a paralogue of SecA (2009), J. Bacteriol., 191, 3482-3491.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.5

Cloned(Commentary)

Cloned (Comment) Organism
subcloned in Escherichia coli Streptococcus gordonii

Protein Variants

Protein Variants Comment Organism
additional information analysis of the function of SecA via mutants and SecA-SecA2-chimeras Streptococcus gordonii
additional information analysis of the function of SecA2 via mutants and SecA-SecA2-chimeras Streptococcus gordonii

Inhibitors

Inhibitors Comment Organism Structure
azide 1.5 mM azide decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA; 1.5 mM azide markedly decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA2 Streptococcus gordonii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Streptococcus gordonii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ ATP hydrolysis activity of the recombinant protein 6His-SecA is highest with 0.1 mM Mg2+ and decreases both with higher and lower concentration of Mg2+ Streptococcus gordonii
Mg2+ ATP hydrolysis activity of the recombinant protein 6His-SecA2 aquires at least 0.5 mM Mg2+ and is higher with 2.5 mM Mg2+ Streptococcus gordonii

Organism

Organism UniProt Comment Textmining
Streptococcus gordonii Q9AET4 SecA
-
Streptococcus gordonii Q9AET6 SecA2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus gordonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 circa 1.8 pMol Pi/pMol 6His-SecA2/min, measured in an assay containing 2.5 mM Mg2+ Streptococcus gordonii
additional information
-
 circa 7 pMol phosphate/pMol 6His-SecA/min measured in an assay containing 0.1 mM Mg2+ Streptococcus gordonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Streptococcus gordonii ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
SecA
-
 Streptococcus gordonii
SecA2 transporter system component specializing in the cell-surface glycoprotein GspB Streptococcus gordonii