Literature summary for 7.4.2.5 extracted from

Janas, E.; Hofacker, M.; Chen, M.; Gompf, S.; van der Does, C.; Tampe, R.
The ATP hydrolysis cycle of the nucleotide-binding domain of the mitochondrial ATP-binding cassette transporter Mdl1p (2003), J. Biol. Chem., 278, 26862-26869.

Search for more literature for 7.4.2.5

Protein Variants

Protein Variants	Comment	Organism
E599Q	3000fold reduction of ATPase activity compared to wild-type	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	ATP	pH 9.0, 30°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	2 * 34000, SDS-PAGE	Saccharomyces cerevisiae
66000	-	gel filtration in presence of MgATP2- and BeFx or orthovanadate	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	recombinant protein expressed in Escherichia coli	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein expressed in Escherichia coli	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + peptide/out	ATP best substrate	Saccharomyces cerevisiae	ADP + phosphate + peptide/in	-	?
CTP + H2O + peptide/out	affinity for CTP is about 30fold lower compared to ATP	Saccharomyces cerevisiae	CDP + phosphate + peptide/in	-	?
GTP + H2O + peptide/out	affinity for GTP is about 30fold lower compared to ATP	Saccharomyces cerevisiae	GDP + phosphate + peptide/in	-	?
UTP + H2O + peptide/out	affinity for UTP is about 30fold lower compared to ATP	Saccharomyces cerevisiae	UDP + phosphate + peptide/in	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 34000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Mdl1	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25	-	ATP	pH 9.0, 30°C	Saccharomyces cerevisiae

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	9	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)