Literature summary for 7.4.2.5 extracted from

Malle, E.; Zhou, H.; Neuhold, J.; Spitzenberger, B.; Klepsch, F.; Pollak, T.; Bergner, O.; Ecker, G.; Stolt-Bergner, P.
Random mutagenesis of the prokaryotic peptide transporter YdgR identifies potential periplasmic gating residues (2011), J. Biol. Chem., 286, 23121-23131.

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling based on the crystal structure of the Shewanella oneidensis peptide transporter PepTso, identifies Glu56 and Arg305 as potential periplasmic gating residues	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
A123V	partial loss of uptake	Escherichia coli
A264P	partial loss of uptake	Escherichia coli
A285V	complete loss of uptake	Escherichia coli
A303G	partial loss of uptake	Escherichia coli
A68P	complete loss of uptake	Escherichia coli
E56G	complete loss of uptake	Escherichia coli
F197I	change in selectivity	Escherichia coli
F289L	complete loss of uptake	Escherichia coli
F289S	complete loss of uptake	Escherichia coli
F301I	complete loss of uptake	Escherichia coli
G101D	partial loss of uptake	Escherichia coli
G127D	partial loss of uptake	Escherichia coli
G78C	complete loss of uptake	Escherichia coli
G86R	partial loss of uptake	Escherichia coli
I100V	no membrane localization	Escherichia coli
I122N	partial loss of uptake	Escherichia coli
I60N	complete loss of uptake	Escherichia coli
K274I	change in selectivity	Escherichia coli
L136R	complete loss of uptake	Escherichia coli
L137H	no membrane localization	Escherichia coli
L190V	change in selectivity	Escherichia coli
L324V	change in selectivity	Escherichia coli
L98R	complete loss of uptake	Escherichia coli
M154K	change in selectivity	Escherichia coli
M295K	complete loss of uptake	Escherichia coli
additional information	screening of a library of mutants to identify amino acids critical for peptide transport and identification of 35 single point mutations that result in a full or partial loss of transport activity	Escherichia coli
N196K	partial loss of uptake	Escherichia coli
N300I	complete loss of uptake	Escherichia coli
N300Y	partial loss of uptake	Escherichia coli
N306I	partial loss of uptake	Escherichia coli
P326Q	partial loss of uptake	Escherichia coli
Q320L	complete loss of uptake	Escherichia coli
R305C	partial loss of uptake	Escherichia coli
S59P	complete loss of uptake	Escherichia coli
T297A	complete loss of uptake	Escherichia coli
V252E	change in selectivity	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
alafosfalin	-	Escherichia coli
L-Ala-L-Ala	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P77304	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/in	-	Escherichia coli	ADP + phosphate + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/out	-	?

Synonyms

Synonyms	Comment	Organism
YdgR	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.05	-	alafosfalin	mutant K274I, pH 7.5, temperature not specified in the publication	Escherichia coli
0.07	-	alafosfalin	wild-type, pH 7.5, temperature not specified in the publication	Escherichia coli
0.08	-	L-Ala-L-Ala	mutant M154K, pH 7.5, temperature not specified in the publication	Escherichia coli
0.2	-	L-Ala-L-Ala	mutant K274I, pH 7.5, temperature not specified in the publication	Escherichia coli
0.2	-	L-Ala-L-Ala	mutant L190V, pH 7.5, temperature not specified in the publication	Escherichia coli
0.21	-	L-Ala-L-Ala	mutant V252E, pH 7.5, temperature not specified in the publication	Escherichia coli
0.21	-	L-Ala-L-Ala	wild-type, pH 7.5, temperature not specified in the publication	Escherichia coli
0.25	-	alafosfalin	mutant M154K, pH 7.5, temperature not specified in the publication	Escherichia coli
0.28	-	alafosfalin	mutant V252E, pH 7.5, temperature not specified in the publication	Escherichia coli
0.31	-	L-Ala-L-Ala	mutant F197I, pH 7.5, temperature not specified in the publication	Escherichia coli
0.36	-	L-Ala-L-Ala	mutant L324V, pH 7.5, temperature not specified in the publication	Escherichia coli
0.38	-	alafosfalin	mutant L190V, pH 7.5, temperature not specified in the publication	Escherichia coli
0.45	-	alafosfalin	mutant F197I, pH 7.5, temperature not specified in the publication	Escherichia coli
0.96	-	alafosfalin	mutant L324V, pH 7.5, temperature not specified in the publication	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.08	-	mutant K274I, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
0.11	-	wild-type, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
0.12	-	mutant M154K, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.31	-	mutant K274I, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.32	-	mutant L190V, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.33	-	mutant V252E, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.33	-	wild-type, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.39	-	mutant M154K, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
0.44	-	mutant V252E, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
0.48	-	mutant F197I, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.56	-	mutant L324V, pH 7.5, temperature not specified in the publication	Escherichia coli	L-Ala-L-Ala
0.59	-	mutant L190V, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
0.71	-	mutant F197I, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin
1.5	-	mutant L324V, pH 7.5, temperature not specified in the publication	Escherichia coli	alafosfalin