EC Number   |
Protein Variants |
Reference  |
|---|
   3.4.22.70 | DELTA N59 |
trucation of N-terminal 59 amino acids does not affect the activity of the enzyme |
647380, 647385 |
| 3.4.22.70 | C184A |
mutant enzyme can not cleave the LPXTG motif |
647382 |
| 3.4.22.70 | DELTA N59 |
enhanced solubility of the mutant helps the crystallization |
647385 |
| 3.4.22.70 | N98A |
kcat/Km is 1.2fold lower than wild-type value |
665561 |
| 3.4.22.70 | N98Q |
kcat/Km is 1.1fold higher than wild-type value |
665561 |
| 3.4.22.70 | R197A |
kcat/Km is 2769fold lower than wild-type value |
665561 |
| 3.4.22.70 | R197H |
kcat/Km is 610fold lower than wild-type value |
665561 |
| 3.4.22.70 | R197K |
kcat/Km is 2571fold lower than wild-type value |
665561 |
| 3.4.22.70 | D170A |
turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is nearly identical to wild-type value |
665718 |
| 3.4.22.70 | E108A |
turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is 43.8fold lower than wild-type value |
665718 |
