Literature summary for 3.4.22.70 extracted from

Marraffini, L.A.; Ton-That, H.; Zong, Y.; Narayana, S.V.; Schneewind, O.
Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A (2004), J. Biol. Chem., 279, 37763-37770.

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Protein Variants

Protein Variants	Comment	Organism
N98A	kcat/Km is 1.2fold lower than wild-type value	Staphylococcus aureus
N98Q	kcat/Km is 1.1fold higher than wild-type value	Staphylococcus aureus
R197A	kcat/Km is 2769fold lower than wild-type value	Staphylococcus aureus
R197H	kcat/Km is 610fold lower than wild-type value	Staphylococcus aureus
R197K	kcat/Km is 2571fold lower than wild-type value	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00824	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, wild-type enzyme	Staphylococcus aureus
0.00932	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme N98Q	Staphylococcus aureus
0.0097	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme N98A	Staphylococcus aureus
0.01391	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197K	Staphylococcus aureus
0.01597	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197A	Staphylococcus aureus
0.0168	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197H	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-LPETG-Dap(Dnp) + H2O	-	Staphylococcus aureus	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00019	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197K	Staphylococcus aureus
0.00021	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197A	Staphylococcus aureus
0.00098	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme R197H	Staphylococcus aureus
0.29	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme N98A	Staphylococcus aureus
0.3	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, wild-type enzyme	Staphylococcus aureus
0.37	-	Abz-LPETG-Dap(Dnp)	37°C, pH 7.5, mutant enzyme N98Q	Staphylococcus aureus

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Release 2023.1 (January 2023)