EC Number |
Protein Variants |
Reference |
---|
2.4.2.29 | D89A |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
2.4.2.29 | D89C |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
2.4.2.29 | D89E |
about 50% of the activity of the histidine-tagged wild-type enzyme |
644933 |
2.4.2.29 | D89N |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
2.4.2.29 | S90A |
activity of the mutant enzyme is to low to determine Vmax and Km-value |
644934 |
2.4.2.29 | S90C |
30fold increase in Km-value for tRNATyr and 4fold increase in Km-value for guanine |
644934 |
2.4.2.29 | S90F |
mutant enzyme has no detectable solubility and reduced solubility |
644934 |
2.4.2.29 | E235Q |
the mutation has no significant influence on kcat, but Km for preQ1 is drastically increased, while Km for preQ0 seems to be decreased |
688390 |
2.4.2.29 | D264N |
is inactive and incapable of forming the covalent intermediate, while maintaining the ability to bind noncovalently to RNA |
702263 |
2.4.2.29 | Y106F |
crystallises similarly to the wild-type |
703226 |