EC Number   |
Protein Variants |
Reference |
|---|
   2.4.2.29 | S90C |
30fold increase in Km-value for tRNATyr and 4fold increase in Km-value for guanine |
644934 |
| 2.4.2.29 | D89E |
about 50% of the activity of the histidine-tagged wild-type enzyme |
644933 |
| 2.4.2.29 | S90A |
activity of the mutant enzyme is to low to determine Vmax and Km-value |
644934 |
| 2.4.2.29 | Y106F |
crystallises similarly to the wild-type |
703226 |
| 2.4.2.29 | D264N |
is inactive and incapable of forming the covalent intermediate, while maintaining the ability to bind noncovalently to RNA |
702263 |
| 2.4.2.29 | D89A |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
| 2.4.2.29 | D89C |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
| 2.4.2.29 | D89N |
less than 1% of the activity of the histidine-tagged wild-type enzyme |
644933 |
| 2.4.2.29 | S90F |
mutant enzyme has no detectable solubility and reduced solubility |
644934 |
| 2.4.2.29 | K52M |
reduced turnover value. At a concentration of protein of 0.01 mM appears almost exclusively as a homodimer as the wild-type, when the concentration of protein is lowered to a minimal value of 0.001 mM, a substantial proportion of monomer becomes evident |
705164 |
