Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one + tRNAguanine
guanine + tRNA2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
-
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one appears to partition between: 1. normal turnover, 2. inactivation, 3. an alternative processing to an unidentrified fluoride-released product
-
-
?
7-(cyano)-7-deazaguanine + tRNAguanine
guanine + tRNA(7-(cyano)-7-deazaguanine)
-
-
inserted 7-(cyano)-7-deazaguanine is located in the first position of the anticodon
?
7-aminomethyl-7-carbaguanine + [RNAECYMA]-2'-deoxyguanine 34
guanine + [RNAECYMA]-2'-deoxy-pre Q1
-
TGT-RNA covalent intermediate
-
-
?
7-aminomethyl-7-carbaguanine + [RNAECYMA]-guanine
guanine + [RNAECYMA]-pre Q1
-
hairpin RNA corresponding to the tRNAtyr anticodon stem-loop motif, TGT-RNA covalent intermediate
-
-
?
7-aminomethyl-7-carbaguanine + [tRNA]-guanine
guanine + [tRNA]-pre Q1
guanine + tRNAguanine
tRNAguanine + guanine
-
-
-
-
r
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
pre-queuine 0 + tRNA guanine
guanine + tRNA pre-queuine 0
-
-
-
?
pre-queuine 1 + virF minihelix RNA
?
-
-
-
?
pre-queuine 1 + virF mRNA
?
TGT may modulate the translation of VirF via modification of the virF mRNA
-
-
?
[tRNATyr]7-aminomethyl-7-carbaguanine + guanine
[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine
additional information
?
-
7-aminomethyl-7-carbaguanine + [tRNA]-guanine
guanine + [tRNA]-pre Q1
-
the TGT-RNA covalent complex is kinetically capable of occurring on the TGT reaction pathway. Dissociation of product RNA from the enzyme is overall rate-limiting in the steady state. Role for the 2'-hydroxyl group of the ribose in the TGT reaction
-
-
?
7-aminomethyl-7-carbaguanine + [tRNA]-guanine
guanine + [tRNA]-pre Q1
Tyr106 in the active site is hydrogen bonded in the ligand-free structure
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
E. coli preQ0-tRNATyr, containing7-(cyano)-7-deazaguanine in the anticodon and E. coli guaninetRNATyr containing guanosine in the anticodon are good acceptors
-
ir
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
the enzyme exchanges the genetically encoded guanine at position 34 with a queuine precursor 7-(aminomethyl)-7-deazaguanine
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
the enzyme is responsible for the posttranscriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. The enzyme catalyzes base exchange of guanosine34 with 7-aminomethyl-7-deazaguanine
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
the enzyme is involved in the hypermodification of cognate tRNAs, leading to the exchange of G34 by 7-methylamino-7-deazaguanine at the wobble position in the anticodon loop
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
prefers pre-queuine 1 over pre-queuine 0
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
?
[tRNATyr]7-aminomethyl-7-carbaguanine + guanine
[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine
-
-
-
?
[tRNATyr]7-aminomethyl-7-carbaguanine + guanine
[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine
TGT active site exhibits a hydrophobic patch formed by Val45 and Leu68, where the phosphate, linking ribose-34 to ribose-35, in bound tRNA is located
-
-
?
[tRNATyr]7-aminomethyl-7-carbaguanine + guanine
[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine
Tgt dimer binds specifically to a single tRNA molecule
-
-
?
additional information
?
-
-
post-translational modification of tRNA
-
-
?
additional information
?
-
-
formation of queuosine
-
-
?
additional information
?
-
-
higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
-
-
?
additional information
?
-
-
the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,3-Dihydroxybenzoic acid
-
2,6-bis(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
2,6-diamino-8-(2-phenylethyl)quinazolin-4(3H)-one
-
2,6-diaminoquinazolin-4(3H)-one
2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2-([2-(morpholin-4-yl)ethyl]amino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
-
inactivation and competitive inhibition. 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one appears to partition between: 1. normal turnover, 2. inactivation, 3. an alternative processing to an unidentrified fluoride-released product
2-amino-7-(dimethylamino)quinazolin-4(3H)-one
-
2-amino-7-[benzyl(methyl)amino]quinazolin-4(3H)-one
-
2-amino-8-methylquinazolin-4(3H)-one
2-aminopteridin-4(3H)-one
-
-
2-aminoquinazolin-4(3H)-one
2-methylamino-lin-benzoguanine
-
2-[(2-phenylethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2-[(thiophen-2-ylmethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
3,5-diaminophthalhydrazide
-
4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
4-(2-[(cyclohexylmethyl)amino]ethyl)2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
4-(2-[(cyclopentylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide
-
5-amino-3-(5-amino-1H-[1,2,4]triazol-3-yl-sulfanyl)-phthalhydrazide
-
6-(ethylamino)-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(benzylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-2-(methylamino)-3-[(methylamino)methyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-3-[2-(methylamino)ethyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-4-(2-[(1-naphthylmethyl)amino]ethyl)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-4-(4-phenylbutyl)-1,7-dihydro-8H-imidazo-[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-4-[(methylamino)methyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-4-[2-(methylamino)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-([2-(morpholin-4-yl)ethyl]amino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-2-[(1,3-benzodioxol-5-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-2-[(2-phenylethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-2-[(3-piperidin-1-ylpropyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
cannot modify the structure of the dimer interface, prevents the formation of the catalytically active Tgt:tRNA complex, and can disrupt the preformed complex
6-amino-2-[(4-morpholin-4-ylbenzyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-2-[(quinolin-4-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-(2-aminoethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-(2-hydroxyethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-(2-[(cycloheptylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
-
6-amino-4-(2-[(cyclohexylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-(2-[(cyclopentylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
strongest inhibitor
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-[2-(benzylamino)ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
6-amino-4-[2-(methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-[2-[(cyclohexylmethyl)amino]ethyl]-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-[(cyclohexylmethyl)amino]ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
can modify the structure of the dimer interface, prevents the formation of the catalytically active Tgt:tRNA complex, and can disrupt the preformed complex
6-amino-4-[2-[(cyclopentylmethyl)amino]ethyl]-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-[(cyclopentylmethyl)amino]ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
addresses a hydrophobic subpocket close to the dimer interface that may affect quarternary structure formation, prevents the formation of the catalytically active Tgt:tRNA complex, and can disrupt the preformed complex
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
benzimidazole-5-carboxylate
-
dimethylsuberimidate
-
inactivates by cross-linking, tRNA protects
methyl 1-[(dimethylamino)sulfonyl]-2-(methylamino)-5-nitro-1H-benz-imidazol-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benz-imidazol-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-4-(4-phenyl-1-butyn-1-yl)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-4-(4-phenylbutyl)-1Hbenzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-4-vinyl-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-4-[2-(methylamino)ethyl]-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-2-(methylamino)-4-{2-[(1-naphthylmethyl)amino]ethyl}-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-4-(2-hydroxyethyl)-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-4-iodo-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-1-[(dimethylamino)sulfonyl]-4-[2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)ethyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-(2-aminoethyl)-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-(2-[(cycloheptylmethyl)amino]ethyl)-1-[(dimethyl-amino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-(2-[(cyclohexylmethyl)amino]ethyl)-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-(2-[(cyclopentylmethyl)amino]ethyl)-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-(2-[benzyl(methyl)amino]ethyl)-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-[2-(benzylamino)ethyl]-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 5-amino-4-{2-[(cyclohexylcarbonyl)amino]ethyl}-1-[(dimethylamino)sulfonyl]-2-(methylamino)-1H-benzimidazole-6-carboxylate
-
methyl 8-benzyl-3-[(dimethylamino)sulfonyl]-2-(methylamino)-3,6,7,8,9,10-hexahydroimidazo[4,5-g][1,3]benzodiazepine-5-carboxylate
-
N-(2-[6-amino-2-(methylamino)-8-oxo-7,8-dihydro-1H-imidazo[4,5-g]quinazolin-4-yl]ethyl)cyclohexanecarboxamide
-
additional information
-
design, synthesis, and in vitro evaluation of novel tricyclic TGT inhibitors based on the lin-benzoguanine scaffold
-
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
2,6-diaminoquinazolin-4(3H)-one
-
-
2,6-diaminoquinazolin-4(3H)-one
-
2-amino-8-methylquinazolin-4(3H)-one
-
-
2-amino-8-methylquinazolin-4(3H)-one
-
2-aminoquinazolin-4(3H)-one
-
-
2-aminoquinazolin-4(3H)-one
-
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
can modify the structure of the dimer interface, prevents the formation of the catalytically active Tgt:tRNA complex, and can disrupt the preformed complex
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-(benzylamino)ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
6-amino-4-[2-(benzylamino)ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
addresses a hydrophobic subpocket close to the dimer interface that may affect quarternary structure formation, prevents the formation of the catalytically active Tgt:tRNA complex, and can disrupt the preformed complex
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
mixed competitive-uncompetitive inhibition with respect to tRNA binding
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
-
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Breast Neoplasms
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
Carcinogenesis
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
Colonic Neoplasms
Absence of tRNA-guanine transglycosylase in a human colon adenocarcinoma cell line.
Colonic Neoplasms
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer.
Dysentery, Bacillary
A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase.
Dysentery, Bacillary
An Immucillin-based Transition State-Analogous Inhibitor of tRNA-Guanine Transglycosylase (TGT).
Dysentery, Bacillary
Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT.
Dysentery, Bacillary
Fragment Screening Hit Draws Attention to a Novel Transient Pocket Adjacent to the Recognition Site of the tRNA-Modifying Enzyme TGT.
Dysentery, Bacillary
High-Affinity Inhibitors of tRNA-Guanine Transglycosylase Replacing the Function of a Structural Water Cluster.
Dysentery, Bacillary
High-affinity inhibitors of Zymomonas mobilis tRNA-guanine transglycosylase through convergent optimization.
Dysentery, Bacillary
How to replace the residual solvation shell of polar active site residues to achieve nanomolar inhibition of tRNA-guanine transglycosylase.
Dysentery, Bacillary
Launching Spiking Ligands into a Protein-Protein Interface: A Promising Strategy to Destabilize and Break Interface Formation in a tRNA Modifying Enzyme.
Dysentery, Bacillary
Replacement of water molecules in a phosphate binding site by furanoside-appended lin-benzoguanine ligands of tRNA-guanine transglycosylase (TGT).
Dysentery, Bacillary
Soaking suggests "alternative facts": Only co-crystallization discloses major ligand-induced interface rearrangements of a homodimeric tRNA-binding protein indicating a novel mode-of-inhibition.
Dysentery, Bacillary
Structure-based drug design: exploring the proper filling of apolar pockets at enzyme active sites.
Dysentery, Bacillary
Swapping Interface Contacts in the Homodimeric tRNA-Guanine Transglycosylase: An Option for Functional Regulation.
Dysentery, Bacillary
Targeting a Cryptic Pocket in a Protein-Protein Contact by Disulfide-Induced Rupture of a Homodimeric Interface.
Dysentery, Bacillary
Unraveling a Ligand-Induced Twist of a Homodimeric Enzyme by Pulsed Electron-Electron Double Resonance.
Foodborne Diseases
High-affinity inhibitors of Zymomonas mobilis tRNA-guanine transglycosylase through convergent optimization.
Foodborne Diseases
Replacement of water molecules in a phosphate binding site by furanoside-appended lin-benzoguanine ligands of tRNA-guanine transglycosylase (TGT).
Liver Neoplasms, Experimental
Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme.
Multiple Sclerosis
The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.
Neoplasms
Characterization and analysis of oncofetal tRNA and its possible application for cancer diagnosis and therapy.
Neoplasms
Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme.
Neoplasms
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
trna-guanosine34 preq1 transglycosylase
deficiency
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0041
(6-aminoimidazol[4,5-g]quinazolin-8(7H)-one)
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
3
2,3-Dihydroxybenzoic acid
-
0.02805
2,6-bis(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.000077
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.0026
2,6-diamino-8-(2-phenylethyl)quinazolin-4(3H)-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.0006 - 0.0015
2,6-diaminoquinazolin-4(3H)-one
0.0065
2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0041
2-([2-(morpholin-4-yl)ethyl]amino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.114 - 0.136
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
0.0031
2-amino-7-(dimethylamino)quinazolin-4(3H)-one
-
0.0041
2-amino-7-[benzyl(methyl)amino]quinazolin-4(3H)-one
-
0.001 - 0.0191
2-amino-8-methylquinazolin-4(3H)-one
0.0022
2-aminopteridin-4(3H)-one
-
37°C, pH 7.3
0.0017 - 0.0021
2-aminoquinazolin-4(3H)-one
0.000058
2-methylamino-lin-benzoguanine
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.0037
2-[(2-phenylethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0029
2-[(thiophen-2-ylmethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0002
3,5-diaminophthalhydrazide
-
0.0021
3,5-diaminophthalimide
-
0.0028
4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3
0.0011
4-(2-[(cyclohexylmethyl)amino]ethyl)2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.00074
4-(2-[(cyclopentylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0083
4-aminophthalhydrazide
-
0.00048
4-aminophthalimide
-
0.038
5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide
-
0.04083
6-(ethylamino)-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.00076
6-amino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.00007
6-amino-2-(benzylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000058
6-amino-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.000058
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.000105
6-amino-2-(methylamino)-4-(2-[(1-naphthylmethyl)amino]ethyl)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000235
6-amino-2-(methylamino)-4-(4-phenylbutyl)-1,7-dihydro-8H-imidazo-[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000026
6-amino-2-(methylamino)-4-[2-(methylamino)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000006
6-amino-2-([2-(morpholin-4-yl)ethyl]amino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0015 - 0.0016
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.000035
6-amino-2-[(1,3-benzodioxol-5-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000006
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.00001
6-amino-2-[(2-phenylethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.00001
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.00004
6-amino-2-[(4-morpholin-4-ylbenzyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000055
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.000027
6-amino-2-[(quinolin-4-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000035
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.000035
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.0073
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.015
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.000055
6-amino-4-(2-aminoethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000097
6-amino-4-(2-hydroxyethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.001 - 0.05
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.0000025
6-amino-4-(2-[(cycloheptylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000004
6-amino-4-(2-[(cyclohexylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000002
6-amino-4-(2-[(cyclopentylmethyl)amino]ethyl)-2-(methylamino)-1,7-dihydro-8Himidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.029
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.0037
6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.0069
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
0.000025
6-amino-4-[2-(benzylamino)ethyl]-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.0037
6-amino-4-[2-(methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.000004
6-amino-4-[2-[(cyclohexylmethyl)amino]ethyl]-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.000002
6-amino-4-[2-[(cyclopentylmethyl)amino]ethyl]-2-(methylamino)-1,4a,7,8a-tetrahydro-8H-imidazo[4,5-g]quinazolin-8-one
pH 7.3, 37°C
0.0041 - 0.0079
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
0.0014
N-(2-[6-amino-2-(methylamino)-8-oxo-7,8-dihydro-1H-imidazo[4,5-g]quinazolin-4-yl]ethyl)cyclohexanecarboxamide
at 37°C, pH 7.3, in 200 mM HEPES buffer, 20 mM MgCl2, 2.95 mM Tween 20 containing both substrates
0.000077
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000077
2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.0006
2,6-diaminoquinazolin-4(3H)-one
-
37°C, pH 7.3
0.0015
2,6-diaminoquinazolin-4(3H)-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.114
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
-
,competitive inhibition
0.136
2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
-
inactivation
0.001
2-amino-8-methylquinazolin-4(3H)-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.0191
2-amino-8-methylquinazolin-4(3H)-one
-
37°C, pH 7.3
0.0017
2-aminoquinazolin-4(3H)-one
-
37°C, pH 7.3
0.0021
2-aminoquinazolin-4(3H)-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.000058
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000058
6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.0015
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.0016
6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000006
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000006
6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.00001
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.00001
6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000055
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000055
6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000035
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.000035
6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
0.0073
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.0073
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.015
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.015
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.001
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.05
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.029
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.029
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.0069
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.0069
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2
0.0041
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
0.0041
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
37°C, pH 7.3, Kic
0.0079
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
37°C, pH7.3, Kiu
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
apoenzyme and in complex with queuine, sitting or hanging drop vapor diffusion method, using 100 mM Tris pH 7.8, 200 mM KBr, 200 mM KSCN, 3% (w/v) polyglutamic acid-LM, 5% (w/v) PEG 4000
2-aminolin-benzoguanine inhibitors in complex with TGT, by hanging-drop, vapor diffusion method at 0°C and macro-seeding, to 1.28-1.78 A resolution, crystals belong to space group C2. The 2-amino-lin-benzoguanines are protonated upon binding to TGT. At pH 5.5, Asp102 is rotated to 75% into the guanine binding pocket whereas at pH 8.5 the same residue is oriented to 100% out of the pocket. Pronounced disorder of the attached side chains addressing the ribose 33 binding pocket
enzyme-inhibitor complexes with 3,5-diaminophthalhydrazide, 4-aminophthalhydrazide, 5-aminonaphthalene-2,3-hydrazide, 5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide, 5-amino-3-(5-amino-1H-[1,2,4]triazol-3-yl-sulfanyl)-phthalhydrazide. 1.95 A crystal structure of 4-aminophthalhydrazide in complex with the enzyme
in complex with guanine, pre-queuine 0, and pre-queuine 1, hanging drop vapour diffusion method, in 100 mM MES, pH 5.5, 1 mM dithiothreitol, 13% (w/v) PEG 8000, 10% (v/v) dimethyl sulfoxide
in complex with linbenzoguanine (6-aminoimidazol[4,5-g]quinazolin-8(7H)-one), hanging drop vapour diffusion method, in with 100 mM MES buffer (pH 5.5), 1 mM dithiothreitol, 8% (w/v) PEG 8000, 10% DMSO, at 20°C
mutant K52M, by hanging-drop, vapor-diffusion method and followed by macroseeding, at a resolution of 2.0 A, forms crystals under the same conditions as wild-type, while all attempts to obtain diffracting crystals from mutant Y330F are unsuccessful. Compared to wild-type, crystals of mutant K52M are very fragile and show only a limited diffraction quality
TGT in complex with 6-amino-4-{2-[(cyclohexylmethyl)amino]ethyl}-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one, residues Val45 and Leu68 form a hydrophobic surface that hosts the cyclohexane ring
wild-type at pH 5.5 to 1.9 A resolution and in complex with pre-queuine 0 to 1.7 A resolution (both crystals belong to space group C2), and in complex with its natural substrate pre-queuine 1 to 2.4 A resolution (crystal belongs to space group C2221). Mutant Y106F alone to 1.95 A resolution and in complex with pre-queuine 1 to 1.9 A resolution (both crystals belong to space group C2). By macroseeding and the hanging-drop method
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Okada, N.; Noguchi, S.; Kasai, H.; Shindo-Okada, N.; Ohgi, T.; Goto, T.; Nishimura, S.
Novel mechanism of post-transcriptional modification of tRNA. Insertion of bases of Q precursors into tRNA by a specific tRNA transglycosylase reaction
J. Biol. Chem.
254
3067-3073
1979
Escherichia coli
brenda
Garcia, G.A.; Koch, K.A.; Chong, S.
tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure
J. Mol. Biol.
231
489-497
1993
Escherichia coli
brenda
Carbon, P.; Haumont, E.; Fournier, M.; de Henau, S.; Grosjean, H.
Site-directed in vitro replacement of nucleosides in the anticodon loop of tRNA: application to the study of structural requirements for queuine insertase activity
EMBO J.
2
1093-1097
1983
Xenopus laevis
brenda
Romier, C.; Meyer, J.E.W.; Suck, D.
Slight sequence variations of a common fold explain the substrate specificities of tRNA-guanine transglycosylases from the three kingdoms
FEBS Lett.
416
93-98
1997
Synechocystis sp., Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Thermotoga maritima
brenda
Brenk, R.; Naerum, L.; Graedler, U.; Gerber, H.D.; Garcia, G.A.; Reuter, K.; Stubbs, M.T.; Klebe, G.
Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis
J. Med. Chem.
46
1133-1143
2003
Shigella flexneri
brenda
Ishiwata, S.; Katayama, J.; Shindo, H.; Ozawa, Y.; Itoh, K.; Mizugaki, M.
Increased expression of queuosine synthesizing enzyme, tRNA-guanine transglycosylase, and queuosine levels in tRNA of leukemic cells
J. Biochem.
129
13-17
2001
Homo sapiens
brenda
Romier, C.; Reuter, K.; Suck, D.; Ficner, R.
Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange
EMBO J.
15
2850-2857
1996
Zymomonas mobilis
brenda
Kittendorf, J.D.; Barcomb, L.M.; Nonekowski, S.T.; Garcia, G.A.
tRNA-guanine transglycosylase from Escherichia coli: Molecular mechanism and role of aspartate 89
Biochemistry
40
14123-14133
2001
Escherichia coli
brenda
Reuter, K.; Chong, S.; Ullrich, F.; Kersten, H.; Garcia, G.A.
Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli
Biochemistry
33
7041-7046
1994
Escherichia coli
brenda
Hoops, G.C.; Townsend, L.B.; Garcia, G.A.
Mechanism-based inactivation of tRNA-guanine transglycosylase from Escherichia coli by 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one
Biochemistry
34
15539-15544
1995
Escherichia coli
brenda
Nonekowski, S.T.; Garcia, G.A.
tRNA recognition by tRNA-guanine transglycosylase from escherichia coli: the role of U33 in U-G-U sequence recognition
RNA
7
1432-1441
2001
Escherichia coli
brenda
Ishiwata, S.; Ozawa, Y.; Katayama, J.; Kaneko, S.; Shindo, H.; Tomioka, Y.; Ishiwata, T.; Asano, G.; Ikegawa, S.; Mizugaki, M.
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer
Cancer Lett.
212
113-119
2004
Homo sapiens
brenda
Stengl, B.; Reuter, K.; Klebe, G.
Mechanism and substrate specificity of tRNA-guanine transglycosylases (TGTs): tRNA-modifying enzymes from the three different kingdoms of life share a common catalytic mechanism
Chembiochem
6
1926-1939
2005
Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Zymomonas mobilis (P28720)
brenda
Meyer, E.A.; Donati, N.; Guillot, M.; Schweizer, W.B.; Diederich, F.; Stengl, B.; Brenk, R.; Reuter, K.; Klebe, G.
Synthesis, biological evaluation, and crystallographic studies of extended guanine-based (lin-benzoguanine) inhibitors for tRNA-guanine transglycosylase (TGT)
Helv. Chim. Acta
89
573-597
2006
Zymomonas mobilis
-
brenda
Stengl, B.; Meyer, E.A.; Heine, A.; Brenk, R.; Diederich, F.; Klebe, G.
Crystal structures of tRNA-guanine transglycosylase (TGT) in complex with novel and potent inhibitors unravel pronounced induced-fit adaptations and suggest dimer formation upon substrate binding
J. Mol. Biol.
370
492-511
2007
Zymomonas mobilis, Zymomonas mobilis (P28720)
brenda
Hoertner, S.R.; Ritschel, T.; Stengl, B.; Kramer, C.; Schweizer, W.B.; Wagner, B.; Kansy, M.; Klebe, G.; Diederich, F.
Potent inhibitors of tRNA-guanine transglycosylase, an enzyme linked to the pathogenicity of the Shigella bacterium: charge-assisted hydrogen bonding
Angew. Chem.
46
8266-8269
2007
Shigella flexneri (Q54177)
brenda
Tidten, N.; Stengl, B.; Heine, A.; Garcia, G.A.; Klebe, G.; Reuter, K.
Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies
J. Mol. Biol.
374
764-776
2007
Zymomonas mobilis (P28720), Zymomonas mobilis
brenda
Hurt, J.K.; Olgen, S.; Garcia, G.A.
Site-specific modification of Shigella flexneri virF mRNA by tRNA-guanine transglycosylase in vitro
Nucleic Acids Res.
35
4905-4913
2007
Escherichia coli (P0A847), Escherichia coli
brenda
Garcia, G.A.; Chervin, S.M.; Kittendorf, J.D.
Identification of the rate-determining step of tRNA-guanine transglycosylase from Escherichia coli
Biochemistry
48
11243-11251
2009
Escherichia coli
brenda
Ritschel, T.; Hoertner, S.; Heine, A.; Diederich, F.; Klebe, G.
Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT
ChemBioChem
10
716-727
2009
Zymomonas mobilis (P28720)
brenda
Kohler, P.C.; Ritschel, T.; Schweizer, W.B.; Klebe, G.; Diederich, F.
High-affinity inhibitors of tRNA-guanine transglycosylase replacing the function of a structural water cluster
Chemistry
15
10809-10817
2009
Zymomonas mobilis (P28720)
brenda
Ritschel, T.; Kohler, P.C.; Neudert, G.; Heine, A.; Diederich, F.; Klebe, G.
How to replace the residual solvation shell of polar active site residues to achieve nanomolar inhibition of tRNA-guanine transglycosylase
ChemMedChem
4
2012-2023
2009
Zymomonas mobilis (P28720)
brenda
Ritschel, T.; Atmanene, C.; Reuter, K.; Van Dorsselaer, A.; Sanglier-Cianferani, S.; Klebe, G.
An integrative approach combining noncovalent mass spectrometry, enzyme kinetics and X-ray crystallography to decipher Tgt protein-protein and protein-RNA interaction
J. Mol. Biol.
393
833-847
2009
Zymomonas mobilis (P28720)
brenda
Barandun, L.J.; Immekus, F.; Kohler, P.C.; Tonazzi, S.; Wagner, B.; Wendelspiess, S.; Ritschel, T.; Heine, A.; Kansy, M.; Klebe, G.; Diederich, F.
From lin-benzoguanines to lin-benzohypoxanthines as ligands for Zymomonas mobilis tRNA-guanine transglycosylase: replacement of protein-ligand hydrogen bonding by importing water clusters
Chemistry
18
9246-9257
2012
Zymomonas mobilis (P28720), Zymomonas mobilis
brenda
Nomura, Y.; Onda, Y.; Ohno, S.; Taniguchi, H.; Ando, K.; Oka, N.; Nishikawa, K.; Yokogawa, T.
Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans
Protein Expr. Purif.
88
13-19
2013
Methanosarcina acetivorans (Q8THU2 and Q8TUE6), Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834 (Q8THU2 and Q8TUE6)
brenda
Sawhney, B.; Chopra, K.; Misra, R.; Ranjan, A.
Identification of Plasmodium falciparum apicoplast-targeted tRNA-guanine transglycosylase and its potential inhibitors using comparative genomics, molecular modelling, docking and simulation studies
J. Biomol. Struct. Dyn.
33
2404-2420
2015
Plasmodium falciparum
brenda
Johannsson, S.; Neumann, P.; Ficner, R.
Crystal structure of the human tRNA guanine transglycosylase catalytic subunit QTRT1
Biomolecules
8
81
2018
Homo sapiens (Q9BXR0), Homo sapiens
brenda