BRENDA - Reference to 2.4.2.29; Id = 644933

overexpression of D89E, D89D, D89C and D89A in Escherichia coli

Escherichia coli

D89A

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89C

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89E

about 50% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89N

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

0.0001

-

guanine

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

0.00011

-

tRNA

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.00012

-

tRNA

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

0.00039

-

tRNA

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

0.00051

-

tRNA

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

0.00057

-

guanine

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

0.00261

-

guanine

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.00632

-

guanine

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

-

wild-type and mutant enzymes D89E, D89D, D89C and D89A

Escherichia coli

0.59

-

guanine

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.71

-

tRNA

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.75

-

guanine

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

0.93

-

tRNA

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

1.21

-

guanine

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

1.21

-

tRNA

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

5.85

-

tRNA

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

7.57

-

guanine

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

overexpression of D89E, D89D, D89C and D89A in Escherichia coli

Escherichia coli

D89A

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89C

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89E

about 50% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

D89N

less than 1% of the activity of the histidine-tagged wild-type enzyme

Escherichia coli

0.0001

-

guanine

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

0.00011

-

tRNA

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.00012

-

tRNA

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

0.00039

-

tRNA

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

0.00051

-

tRNA

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

0.00057

-

guanine

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

0.00261

-

guanine

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.00632

-

guanine

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

wild-type and mutant enzymes D89E, D89D, D89C and D89A

Escherichia coli

0.59

-

guanine

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.71

-

tRNA

histidine-tagged mutant enzyme D89E, pH 7.3

Escherichia coli

0.75

-

guanine

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

0.93

-

tRNA

histidine-tagged mutant enzyme D89E, pH 8.5

Escherichia coli

1.21

-

guanine

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

1.21

-

tRNA

histidine-tagged wild-type enzyme, pH 7.3

Escherichia coli

5.85

-

tRNA

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

7.57

-

guanine

histidine-tagged wild-type enzyme, pH 8.5

Escherichia coli

735961

Barandun

Replacement of water molecules ...

Zymomonas mobilis

Chemistry

21

126-135

2015

-

8

-

2

-

1

-

2

-

2

-

8

-

8

-

2

-

2

-

736521

Sawhney

Identification of Plasmodium f ...

Plasmodium falciparum

J. Biomol. Struct. Dyn.

33

2404-2420

2015

-

1

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

728740

Nomura

Purification and comparison of ...

Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834

Protein Expr. Purif.

88

13-19

2013

-

1

-

1

-

1

3

-

3

-

1

-

1

2

1

-

1

-

1

-

1

-

1

3

-

1

-

1

-

1

-

1

-

735359

Barandun

High-affinity inhibitors of Zy ...

Zymomonas mobilis

Acta Crystallogr. Sect. D

69

1798-1807

2013

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

722058

Barandun

From lin-benzoguanines to lin- ...

Zymomonas mobilis

Chemistry

18

9246-9257

2012

-

14

-

6

-

2

-

14

-

14

-

721496

Eric Thomas

Differential heterocyclic subs ...

Escherichia coli, Homo sapiens

Biochem. Biophys. Res. Commun.

410

34-39

2011

-

4

-

4

6

-

7

-

2

-

4

2

-

6

2

-

10

-

4

-

4

10

6

-

2

-

2

-

6

2

-

6

706730

Chen

Characterization of the human ...

Homo sapiens

RNA

16

958-968

2010

1

-

1

-

4

-

1

-

1

6

-

1

-

1

-

1

-

1

-

702263

Garcia

Identification of the rate-det ...

Escherichia coli

Biochemistry

48

11243-11251

2009

-

1

-

2

-

1

-

1

-

3

-

2

-

2

-

1

-

2

-

1

-

3

-

2

-

703202

Ritschel

Crystal structure analysis and ...

Zymomonas mobilis

ChemBioChem

10

716-727

2009

-

1

-

10

-

2

-

1

-

1

-

2

-

10

-

1

-

1

-

10

-

1

-

1

-

703213

Kohler

High-affinity inhibitors of tR ...

Zymomonas mobilis

Chemistry

15

10809-10817

2009

-

1

-

30

-

3

-

1

-

2

-

11

-

1

-

30

11

-

1

-

703226

Ritschel

How to replace the residual so ...

Zymomonas mobilis

ChemMedChem

4

2012-2023

2009

-

1

-

4

-

1

-

1

-

2

-

4

-

1

-

4

-

4

-

1

-

4

-

4

705164

Ritschel

An integrative approach combin ...

Zymomonas mobilis

J. Mol. Biol.

393

833-847

2009

-

1

2

-

5

3

-

1

3

-

3

-

1

2

-

3

-

1

-

1

2

-

5

-

3

-

1

3

-

1

-

3

-

681439

Stengl

Crystal structures of tRNA-gua ...

Zymomonas mobilis

J. Mol. Biol.

370

492-511

2007

-

1

-

1

-

18

-

1

-

4

-

1

2

-

17

-

1

-

2

-

18

17

-

1

-

1

-

684430

Hoertner

Potent inhibitors of tRNA-guan ...

Shigella flexneri

Angew. Chem.

46

8266-8269

2007

-

12

-

3

-

1

-

1

-

12

-

12

-

1

-

688390

Tidten

Glutamate versus glutamine exc ...

Zymomonas mobilis

J. Mol. Biol.

374

764-776

2007

-

1

-

8

-

3

-

2

-

1

-

8

-

1

-

1

-

8

-

2

-

8

-

689250

Hurt

Site-specific modification of ...

Escherichia coli

Nucleic Acids Res.

35

4905-4913

2007

-

3

-

6

-

3

-

1

-

3

-

3

-

3

-

3

-

678191

Todorov

Role of aspartate 143 in Esche ...

Escherichia coli

Biochemistry

45

617-625

2006

-

1

10

-

2

-

3

-

2

-

6

-

2

-

1

2

10

-

3

-

6

-

680057

Meyer

-

Synthesis, biological evaluati ...

Zymomonas mobilis

Helv. Chim. Acta

89

573-597

2006

-

13

-

1

-

13

-

13

-

658289

Todorov

The role of aspartic acid 143 ...

Escherichia coli

Biophys. J.

89

1965-1977

2005

-

4

-

1

-

5

-

2

-

1

-

4

-

1

-

2

-

679204

Stengl

Mechanism and substrate specif ...

Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Zymomonas mobilis

Chembiochem

6

1926-1939

2005

-

1

-

4

-

6

-

1

-

658379

Ishiwata

Elevated expression level of 6 ...

Homo sapiens

Cancer Lett.

212

113-119

2004

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

644928

Brenk

Virtual screening for submicro ...

Shigella flexneri

J. Med. Chem.

46

1133-1143

2003

-

1

-

3

-

2

-

1

-

2

-

644939

Goodenough-Lashua

tRNA-guanine transglycosylase ...

Escherichia coli, Shigella flexneri

Bioorg. Chem.

31

331-344

2003

-

1

-

1

-

2

-

5

-

1

-

2

-

1

-

1

-

2

-

2

-

644940

Kittendorf

An essential role for aspartat ...

Escherichia coli, Zymomonas mobilis

J. Biol. Chem.

278

42369-42376

2003

-

1

6

-

6

-

1

-

5

-

1

-

1

-

1

6

-

6

-

1

-

1

-

658446

Brenk

Flexible adaptations in the st ...

Zymomonas mobilis

ChemBioChem

4

1066-1077

2003

-

1

-

4

-

1

-

4

-

2

-

2

-

4

-

1

-

4

-

4

-

1

-

2

-

4

-

644931

Nonekowski

The Escherichia coli tRNA-guan ...

Escherichia coli, Pyrococcus horikoshii

J. Biol. Chem.

277

7178-7182

2002

-

4

-

644929

Ishiwata

Increased expression of queuos ...

Homo sapiens

J. Biochem.

129

13-17

2001

-

1

-

2

-

5

-

1

-

1

-

5

-

1

-

644933

Kittendorf

tRNA-guanine transglycosylase ...

Escherichia coli

Biochemistry

40

14123-14133

2001

-

1

-

4

-

8

-

3

-

1

-

8

-

1

-

4

-

8

-

1

-

8

-

644937

Nonekowski

tRNA recognition by tRNA-guani ...

Escherichia coli

RNA

7

1432-1441

2001

-

1

-

3

-

2

-

1

-

2

-

644938

Kung

tRNA-guanine transglycosylase ...

Escherichia coli

RNA

6

233-244

2000

-

6

-

1

-

1

-

644926

Kung

tRNA-guanine transglycosylase ...

Escherichia coli

FEBS Lett.

431

427-432

1998

-

1

-

4

-

1

-

1

-

1

-

644927

Romier

Slight sequence variations of ...

Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Synechocystis sp., Thermotoga maritima

FEBS Lett.

416

93-98

1997

-

8

-

8

-

8

-

644930

Romier

Crystal structure of tRNA-guan ...

Zymomonas mobilis

EMBO J.

15

2850-2857

1996

-

1

-

3

-

1

-

1

-

1

-

644936

Deshpande

Cloning and characterization o ...

Oryctolagus cuniculus

Arch. Biochem. Biophys.

326

1-7

1996

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

644932

Curnow

tRNA-guanine transglycosylase ...

Escherichia coli

J. Biol. Chem.

270

17264-17267

1995

-

4

-

1

-

1

-

644935

Hoops

Mechanism-based inactivation o ...

Escherichia coli

Biochemistry

34

15539-15544

1995

-

1

-

3

-

1

-

1

-

2

-

1

2

1

-

1

-

1

-

644934

Reuter

Serine 90 is required for enzy ...

Escherichia coli

Biochemistry

33

7041-7046

1994

-

1

-

3

-

4

-

1

-

2

-

1

-

2

-

1

-

3

-

4

-

1

-

1

-

2

-

644920

Garcia

tRNA-guanine transglycosylase ...

Escherichia coli

J. Mol. Biol.

231

489-497

1993

-

1

-

2

-

1

-

2

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

644919

Gunduz

-

Transfer -RNA guanine transgly ...

Salmonella enterica subsp. enterica serovar Typhimurium

Period. Biol.

90

321-326

1988

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

644922

Haumont

Enzymatic formation of queuosi ...

Xenopus laevis

Eur. J. Biochem.

168

219-225

1987

-

4

-

1

-

2

-

1

-

2

-

644921

Farkas

Substrate and inhibitor specif ...

Oryctolagus cuniculus

Biochim. Biophys. Acta

781

64-75

1984

-

20

-

4

-

1

-

6

-

7

-

20

7

-

1

-

6

-

644924

Carbon

Site-directed in vitro replace ...

Xenopus laevis

EMBO J.

2

1093-1097

1983

-

3

-

1

-

1

-

644923

Walden

Purification and properties of ...

Triticum aestivum

J. Biol. Chem.

257

13218-13222

1982

-

8

1

-

9

2

-

1

-

1

-

1

-

3

1

-

1

-

1

-

8

1

-

9

2

-

1

-

1

-

3

1

-

1

-

644925

Shindo-Okada

Transfer ribonucleic acid guan ...

Rattus norvegicus

Biochemistry

19

395-400

1980

-

2

-

4

-

1

-

2

1

-

7

-

2

-

1

-

2

1

-

7

-

644917

Okada

Novel mechanism of post-transc ...

Escherichia coli

J. Biol. Chem.

254

3067-3073

1979

-

2

-

2

-

3

-

5

-

2

-

2

-

5

-

644918

Howes

Studies with a homogeneous enz ...

Oryctolagus cuniculus

J. Biol. Chem.

253

9082-9087

1978

-

1

-

2

1

-

3

1

-

1

-

1

-

1

2

1

-

1

-

2

1

-

3

1

-

1

-

1

2

1

-

tRNA-guanine transglycosylase from Escherichia coli: Molecular mechanism and role of aspartate 89

Data extracted from this reference: