Cloned (Comment) | Organism |
---|---|
overexpression of D89E, D89D, D89C and D89A in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D89A | less than 1% of the activity of the histidine-tagged wild-type enzyme | Escherichia coli |
D89C | less than 1% of the activity of the histidine-tagged wild-type enzyme | Escherichia coli |
D89E | about 50% of the activity of the histidine-tagged wild-type enzyme | Escherichia coli |
D89N | less than 1% of the activity of the histidine-tagged wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
guanine | histidine-tagged wild-type enzyme, pH 7.3 | Escherichia coli | |
0.00011 | - |
tRNA | histidine-tagged mutant enzyme D89E, pH 7.3 | Escherichia coli | |
0.00012 | - |
tRNA | histidine-tagged wild-type enzyme, pH 7.3 | Escherichia coli | |
0.00039 | - |
tRNA | histidine-tagged wild-type enzyme, pH 8.5 | Escherichia coli | |
0.00051 | - |
tRNA | histidine-tagged mutant enzyme D89E, pH 8.5 | Escherichia coli | |
0.00057 | - |
guanine | histidine-tagged wild-type enzyme, pH 8.5 | Escherichia coli | |
0.00261 | - |
guanine | histidine-tagged mutant enzyme D89E, pH 7.3 | Escherichia coli | |
0.00632 | - |
guanine | histidine-tagged mutant enzyme D89E, pH 8.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes D89E, D89D, D89C and D89A | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
guanine | histidine-tagged mutant enzyme D89E, pH 7.3 | Escherichia coli | |
0.71 | - |
tRNA | histidine-tagged mutant enzyme D89E, pH 7.3 | Escherichia coli | |
0.75 | - |
guanine | histidine-tagged mutant enzyme D89E, pH 8.5 | Escherichia coli | |
0.93 | - |
tRNA | histidine-tagged mutant enzyme D89E, pH 8.5 | Escherichia coli | |
1.21 | - |
guanine | histidine-tagged wild-type enzyme, pH 7.3 | Escherichia coli | |
1.21 | - |
tRNA | histidine-tagged wild-type enzyme, pH 7.3 | Escherichia coli | |
5.85 | - |
tRNA | histidine-tagged wild-type enzyme, pH 8.5 | Escherichia coli | |
7.57 | - |
guanine | histidine-tagged wild-type enzyme, pH 8.5 | Escherichia coli |