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Literature summary for 2.4.2.29 extracted from

  • Reuter, K.; Chong, S.; Ullrich, F.; Kersten, H.; Garcia, G.A.
    Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli (1994), Biochemistry, 33, 7041-7046.
    View publication on PubMed
Search for more literature for 2.4.2.29

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes S90F and S90C Escherichia coli

Protein Variants

Protein Variants Comment Organism
S90A activity of the mutant enzyme is to low to determine Vmax and Km-value Escherichia coli
S90C 30fold increase in Km-value for tRNATyr and 4fold increase in Km-value for guanine Escherichia coli
S90F mutant enzyme has no detectable solubility and reduced solubility Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 tRNA(Tyr) wild-type enzyme Escherichia coli
0.0011
-
 guanine mutant enzyme S90A Escherichia coli
0.003
-
 guanine wild-type enzyme Escherichia coli
0.0097
-
 tRNA(Tyr) mutant enzyme S90C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine Escherichia coli the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme S90F, S90C and S90A Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
-
 Escherichia coli 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr Escherichia coli 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?