EC Number   |
Protein Variants |
Reference |
|---|
   4.2.2.7 | H202A |
no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |
| 4.2.2.7 | H202A/Y257A |
His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |
| 4.2.2.7 | H406A |
no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |
| 4.2.2.7 | I133T/P316T |
mutant displays about 7fold increase in catalytic efficiency |
747009 |
| 4.2.2.7 | K185A |
about 100% of wild-type activity |
-, 704591 |
| 4.2.2.7 | K252A |
about 40% of wild-type activity |
704591 |
| 4.2.2.7 | K353A |
no activity |
-, 704591 |
| 4.2.2.7 | more |
construction of several double and triple mutants of the calcium-coordinating residues |
651971 |
| 4.2.2.7 | N375A |
produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments |
651971 |
| 4.2.2.7 | N405A |
site-directed mutagenesis, compared to the wild-type enzyme, the mutant shows broadened substrate specificity and cleaves the resistant linkage proximate to the 3-O-sulfoglucosamine residue |
715037 |
