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Literature summary for 4.2.2.7 extracted from

  • Shaya, D.; Zhao, W.; Garron, M.L.; Xiao, Z.; Cui, Q.; Zhang, Z.; Sulea, T.; Linhardt, R.J.; Cygler, M.
    Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate (2010), J. Biol. Chem., 285, 20051-20061.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pedobacter heparinus Pedobacter heparinus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Pedobacter heparinus

Protein Variants

Protein Variants Comment Organism
H202/H406A His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
H202A no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
H202A/Y257A His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
H406A no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
Y257A no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
Y257A/H406A His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus
Y257F no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
2 * 85000, calculated: 84545 Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography (SP-Sepharose), hydroxylapatite chromatography, mutant proteins by immobilized metal-chelate chromatography, His-tag is removed, further purification by ion-exchange chromatography Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acts on heparin and heparan sulfate Pedobacter heparinus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 85000, calculated: 84545 Pedobacter heparinus

Synonyms

Synonyms Comment Organism
heparin lyase II
-
Pedobacter heparinus
heparinase II
-
Pedobacter heparinus
heparitinase II
-
Pedobacter heparinus
HepII
-
Pedobacter heparinus

pI Value

Organism Comment pI Value Maximum pI Value
Pedobacter heparinus
-
-
8.9