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Literature summary for 4.2.2.7 extracted from

  • Yu, P.; Jia, T.; Chen, Y.; Wu, Y.; Zhang, Y.
    Improving the activity of heparinase I by the directed evolution, its enzymatic properties and optimal conditions for heparin degrading by recombinant cells (2016), Biochem. Eng. J., 114, 237-243 .
No PubMed abstract available
Search for more literature for 4.2.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pedobacter heparinus

Protein Variants

Protein Variants Comment Organism
I133T/P316T mutant displays about 7fold increase in catalytic efficiency Pedobacter heparinus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, about 30% inhibition Pedobacter heparinus
Mg2+ 1 mM, about 30% inhibition Pedobacter heparinus
Mn2+ 1 mM, about 30% inhibition Pedobacter heparinus
Ni2+ 1 mM, about 30% inhibition Pedobacter heparinus
Zn2+ 1 mM, about 30% inhibition Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 heparin mutant I133T/P316T, pH 7.5, 40°C Pedobacter heparinus
0.56
-
 heparin wild-type, pH 7.5, 40°C Pedobacter heparinus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 57% increase in activity for mutant I133T/P316T Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus B3U3D9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin
-
 Pedobacter heparinus ?
-
 ?

Synonyms

Synonyms Comment Organism
heparinase I
-
 Pedobacter heparinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 heparin wild-type, pH 7.5, 40°C Pedobacter heparinus
27
-
 heparin mutant I133T/P316T, pH 7.5, 40°C Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pedobacter heparinus

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 almost no activity for wild-type Pedobacter heparinus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.038
-
 heparin wild-type, pH 7.5, 40°C Pedobacter heparinus
0.27
-
 heparin mutant I133T/P316T, pH 7.5, 40°C Pedobacter heparinus