Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Bacteroides thetaiotaomicron |
Crystallization (Comment) | Organism |
---|---|
sitting drop method | Bacteroides thetaiotaomicron |
Protein Variants | Comment | Organism |
---|---|---|
DELTA 156-221 | no activity | Bacteroides thetaiotaomicron |
DELTA 191-213 | about 5% of wild-type activity | Bacteroides thetaiotaomicron |
H151A | no activity | Bacteroides thetaiotaomicron |
K185A | about 100% of wild-type activity | Bacteroides thetaiotaomicron |
K252A | about 40% of wild-type activity | Bacteroides thetaiotaomicron |
K353A | no activity | Bacteroides thetaiotaomicron |
Q149A | no activity | Bacteroides thetaiotaomicron |
Q223A | about 40% of wild-type activity | Bacteroides thetaiotaomicron |
Q22A | about 20% of wild-type activity | Bacteroides thetaiotaomicron |
R156A | about 90% of wild-type activity | Bacteroides thetaiotaomicron |
R83A | no activity | Bacteroides thetaiotaomicron |
Y357A | no activity | Bacteroides thetaiotaomicron |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
heparin, 20-mer | wild-type protein | Bacteroides thetaiotaomicron | |
0.0182 | - |
heparin, 8-mer | wild-type protein | Bacteroides thetaiotaomicron | |
0.0541 | - |
heparin, 6-mer | wild-type protein | Bacteroides thetaiotaomicron | |
0.2395 | - |
heparin, 20-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron | |
5.015 | - |
heparin, 6-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | - |
- |
- |
Bacteroides thetaiotaomicron WAL2926 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography, ion exchange chromatography, gel filtration | Bacteroides thetaiotaomicron |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.8 | - |
heparin (20-mer), deletion mutant 191-213 protein | Bacteroides thetaiotaomicron |
21.1 | - |
heparin (6-mer), deletion mutant 191-213 protein | Bacteroides thetaiotaomicron |
40.6 | - |
heparin (20-mer), wild-type protein | Bacteroides thetaiotaomicron |
101.4 | - |
heparin (8-mer), wild-type protein | Bacteroides thetaiotaomicron |
107.8 | - |
heparin (6-mer), wild-type protein | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
heparin | - |
Bacteroides thetaiotaomicron WAL2926 | ? | - |
? | |
heparin, 20-mer | - |
Bacteroides thetaiotaomicron | unsaturated heparin disaccharides | - |
? | |
heparin, 20-mer | - |
Bacteroides thetaiotaomicron WAL2926 | unsaturated heparin disaccharides | - |
? | |
heparin, 6-mer | - |
Bacteroides thetaiotaomicron | unsaturated heparin disaccharides | - |
? | |
heparin, 6-mer | - |
Bacteroides thetaiotaomicron WAL2926 | unsaturated heparin disaccharides | - |
? | |
heparin, 8-mer | - |
Bacteroides thetaiotaomicron | unsaturated heparin disaccharides | - |
? | |
heparin, 8-mer | - |
Bacteroides thetaiotaomicron WAL2926 | unsaturated heparin disaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heparin lyase I | - |
Bacteroides thetaiotaomicron |
heparinase I | - |
Bacteroides thetaiotaomicron |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
heparin, 20-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron | |
14.5 | - |
heparin, 6-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron | |
29.1 | - |
heparin, 20-mer | wild-type protein | Bacteroides thetaiotaomicron | |
73.7 | - |
heparin, 8-mer | wild-type protein | Bacteroides thetaiotaomicron | |
78.4 | - |
heparin, 6-mer | wild-type protein | Bacteroides thetaiotaomicron |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
heparin, 6-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron | |
16.5 | - |
heparin, 20-mer | deletion mutant 191-213 protein | Bacteroides thetaiotaomicron | |
1448 | - |
heparin, 6-mer | wild-type protein | Bacteroides thetaiotaomicron | |
4054 | - |
heparin, 8-mer | wild-type protein | Bacteroides thetaiotaomicron | |
6311 | - |
heparin, 20-mer | wild-type protein | Bacteroides thetaiotaomicron |