4.2.2.7	D210A	mutation does not affect the product profile compared to the wild type anzyme	651971	
4.2.2.7	D212A	mutation does not affect the product profile compared to the wild type anzyme	651971	
4.2.2.7	DELTA 156-221	no activity	704591	
4.2.2.7	DELTA 191-213	about 5% of wild-type activity	704591	
4.2.2.7	E207A	mutation does not affect the product profile compared to the wild type anzyme	651971	
4.2.2.7	E381A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	G213A	mutation does not affect the product profile compared to the wild type anzyme	651971	
4.2.2.7	G378A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	H151A	no activity	-, 704591	
4.2.2.7	H202/H406A	His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	H202A	no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	H202A/Y257A	His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	H406A	no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	I133T/P316T	mutant displays about 7fold increase in catalytic efficiency	747009	
4.2.2.7	K185A	about 100% of wild-type activity	-, 704591	
4.2.2.7	K252A	about 40% of wild-type activity	704591	
4.2.2.7	K353A	no activity	-, 704591	
4.2.2.7	additional information	construction of several double and triple mutants of the calcium-coordinating residues	651971	
4.2.2.7	N375A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	N405A	site-directed mutagenesis, compared to the wild-type enzyme, the mutant shows broadened substrate specificity and cleaves the resistant linkage proximate to the 3-O-sulfoglucosamine residue	715037	
4.2.2.7	N405A/H406A	site-directed mutagenesis	715037	
4.2.2.7	N405G	site-directed mutagenesis, compared to the wild-type enzyme, the mutant shows broadened substrate specificity and cleaves the resistant linkage proximate to the 3-O-sulfoglucosamine residue	715037	
4.2.2.7	N405G/H406A	site-directed mutagenesis	715037	
4.2.2.7	Q149A	no activity	704591	
4.2.2.7	Q223A	about 40% of wild-type activity	704591	
4.2.2.7	Q22A	about 20% of wild-type activity	-, 704591	
4.2.2.7	R156A	about 90% of wild-type activity	-, 704591	
4.2.2.7	R83A	no activity	704591	
4.2.2.7	S377A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	T216A	mutation does not affect the product profile compared to the wild type anzyme	651971	
4.2.2.7	T373A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	T382A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971	
4.2.2.7	Y257A	no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	Y257A/H406A	His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	Y257F	no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3)	704673	
4.2.2.7	Y357A	no activity	704591	
4.2.2.7	Y379A	produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments	651971