EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.56 | DELTA1-311 |
no significant influence on localization of enzyme |
646399 |
| 3.1.3.56 | DELTA1-433 |
enzyme mainly in cytoplasm |
646399 |
| 3.1.3.56 | DELTA407-411 |
predominantly soluble |
646395 |
| 3.1.3.56 | DELTA725-1001 |
enzyme exclusively cytoplasmic |
646399 |
| 3.1.3.56 | E185A |
site-directed mutagenesis, mutation in the Flex-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | E55A |
no activity |
646400 |
| 3.1.3.56 | E55Q |
no activity |
646400 |
| 3.1.3.56 | E597Q |
site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme |
665575 |
| 3.1.3.56 | E631A |
no activity |
646400 |
| 3.1.3.56 | F246A |
site-directed mutagenesis, mutation in the bulky and hydrophobic loop, inactive mutant |
751120 |
