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Literature summary for 3.1.3.56 extracted from

  • Whisstock, J.C.; Romero, S.; Gurung, R.; Nandurkar, H.; Ooms, L.M.; Bottomley, S.P.; Mitchell, C.A.
    The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis (2000), J. Biol. Chem., 275, 37055-37061.
    View publication on PubMed
Search for more literature for 3.1.3.56

Cloned(Commentary)

Cloned (Comment) Organism
His-tag fusion protein expressed in Escherichia coli Mus musculus
His-tag fusion protein expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D232A traces of activity Mus musculus
D232A no activity Mus musculus
D384A no activity Mus musculus
D771A no activity Saccharomyces cerevisiae
E55A no activity Mus musculus
E55Q no activity Mus musculus
E631A no activity Saccharomyces cerevisiae
F392A 50% of activity Mus musculus
H184A traces of activity Mus musculus
H385A no activity Mus musculus
H730A no activity Saccharomyces cerevisiae
K386A no activity Mus musculus
N18A no activity Mus musculus
P387A traces of activity Mus musculus
T16A 100% of activity Mus musculus
V388A traces of activity Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus
Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,4,5-trisphosphate + H2O Mus musculus regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O Saccharomyces cerevisiae regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag fusion protein expressed in Escherichia coli Mus musculus
His-tag fusion protein expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3-sn-phosphatidyl)-L-myo-inositol 4,5-bisphosphate + H2O
-
 Saccharomyces cerevisiae phosphatidylinositol 4-phosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Mus musculus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Saccharomyces cerevisiae D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Mus musculus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Saccharomyces cerevisiae D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?