EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.60 | Q890L |
site-directed mutagenesis, the mutant shows almost abolished 5,8-linoleate diol synthase activity |
724132 |
| 1.13.11.60 | Q898E |
site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product |
741712 |
| 1.13.11.60 | Q898L |
site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product |
741712 |
| 1.13.11.60 | R707A |
the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation |
764229 |
| 1.13.11.60 | R814A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | R934A |
the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation |
764229 |
| 1.13.11.60 | T879A |
the mutant shows more than 110% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | V1010A |
the mutant shows more than 110% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | V330L |
the wild-type enzyme forms 98% (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate and 2% (8E,10R,12Z)-10-hydroperoxy-9,12-octadecadienoate. The V330L mutation augments the formation of (8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate 3fold |
704475 |
| 1.13.11.60 | V884A |
the mutant shows more than 110% activity of the wild type enzyme |
764229 |
