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Literature summary for 1.13.11.60 extracted from

  • Seo, M.J.; Kang, W.R.; Yang, E.J.; Shin, K.C.; Ko, Y.J.; Oh, D.K.
    Molecular characterization of Penicillium oxalicum 6R,8R-linoleate diol synthase with new regiospecificity (2019), Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1864, 577-586 .
    View publication on PubMed
Search for more literature for 1.13.11.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia ER2566 cells Penicillium oxalicum

Protein Variants

Protein Variants Comment Organism
C1005S the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
D749A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
E728A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
F794E the mutant acts as 7,8-linoleate diol synthase Penicillium oxalicum
F794Y the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
F798Y the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
H1003A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
I841A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
L970A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
M1051A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
M695A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
M883A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
N886D the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
P1016A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
Q887A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
Q889A the mutant acts as 8,11-linoleate diol synthase Penicillium oxalicum
Q889A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
R707A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
R814A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
R934A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
T879A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
V1010A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
V884A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
Y690F the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0264
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
0.0283
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
0.0329
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
0.0475
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
370000
-
 gel filtration Penicillium oxalicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Penicillium oxalicum
-
 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
linoleate + O2 Penicillium oxalicum KCTC 6440
-
 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum
-
-
-
Penicillium oxalicum KCTC 6440
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2
-
 Penicillium oxalicum (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid
-
 ?
alpha-linolenic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid
-
 ?
linoleate + O2
-
 Penicillium oxalicum (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
linoleate + O2
-
 Penicillium oxalicum KCTC 6440 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
linoleic acid + O2 highest activity Penicillium oxalicum (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid
-
 ?
linoleic acid + O2 highest activity Penicillium oxalicum KCTC 6440 (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid
-
 ?
oleic acid + O2
-
 Penicillium oxalicum (6R,8R,9Z)-dihydroxy-9-octadecenoic acid
-
 ?
oleic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6R,8R,9Z)-dihydroxy-9-octadecenoic acid
-
 ?
palmitoleic acid + O2
-
 Penicillium oxalicum (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid
-
 ?
palmitoleic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 121000, full-length enzyme, SDS-PAGE Penicillium oxalicum

Synonyms

Synonyms Comment Organism
6,8-diol synthase
-
 Penicillium oxalicum
6R,8R-linoleate diol synthase
-
 Penicillium oxalicum
6R,8RLDS
-
 Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Penicillium oxalicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme exhibits 28% of its initial activity at 50°C after 2 h Penicillium oxalicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.34
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
21.4
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
30.4
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
32.8
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Penicillium oxalicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
450
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1070
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1240
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum