EC Number |
Protein Variants |
Reference |
---|
1.13.11.60 | N895L |
site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme |
-, 741712 |
1.13.11.60 | N895Q |
site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme |
741712 |
1.13.11.60 | N938D |
site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase reduces the hydroperoxide isomerase activity of the enzyme |
724132 |
1.13.11.60 | N938L |
site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase abolishes the hydroperoxide isomerase activity of the enzyme |
724132 |
1.13.11.60 | N938Q |
site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase reduces the hydroperoxide isomerase activity of the enzyme |
724132 |
1.13.11.60 | P1016A |
the mutant shows more than 110% activity of the wild type enzyme |
764229 |
1.13.11.60 | Q887A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
1.13.11.60 | Q889A |
the mutant acts as 8,11-linoleate diol synthase |
-, 764229 |
1.13.11.60 | Q889A |
the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation |
-, 764229 |
1.13.11.60 | Q890E |
site-directed mutagenesis, the mutant retains (5S,8R)-5,8-dihydroxylinoleate as the main product, but shifts oxygenation from C-5 to C-7 and C-11 and to formation of epoxyalcohols by homolytic scission of (8R)-hydroperoxylinoleic acid |
724132 |