BRENDA - Reference to 1.13.11.60; Id = 741712

DMSO

the whole enzyme activity is the highest using DMSO among the organic solvents tested, and its optimal concentration is 2.5% v/v

Colletotrichum gloeosporioides

expression in Escherichia coli

Colletotrichum gloeosporioides

phylogenetic tree, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain ER2566

Colletotrichum gloeosporioides

A891G

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

C1038S

site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE

Colletotrichum gloeosporioides

H1036A

site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE

Colletotrichum gloeosporioides

H1036A/C1038S

site-directed mutagenesis, the double mutant converts linoleic acid to only 8-HPODE

Colletotrichum gloeosporioides

additional information

comparison of substrate specificities of wild-type an d mutant enzymes, overview

Colletotrichum gloeosporioides

N895D

site-directed mutagenesis, the mutant produces a reduced amount of 8-HPODE, compared to wild-type, without the accumulation of DiHODE

Colletotrichum gloeosporioides

N895L

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

N895Q

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

Q898E

site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product

Colletotrichum gloeosporioides

Q898L

site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product

Colletotrichum gloeosporioides

Y380F

site-directed mutagenesis, the mutant enzyme produces trace amount of 7,8-DiHODE without the accumulation of 8-HPODE when incubated with linoleic acid as substrate

Colletotrichum gloeosporioides

0.0193

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0193

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.022

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0264

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0264

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0293

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0293

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0305

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0305

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0319

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0319

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0324

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0324

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0333

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0358

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0358

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0368

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0369

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0371

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.048

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

510000

-

gel filtration

Colletotrichum gloeosporioides

510000

-

recombinant enzyme, gel filtration

Colletotrichum gloeosporioides

linoleate + O2

Colletotrichum gloeosporioides

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

-

?

DMSO

maximum activity in presence of 2.5% DMSO

Colletotrichum gloeosporioides

A0A0S2SWE4

i.e. Colletotrichum gloeosporioides

-

Colletotrichum gloeosporioides

A0A0S2SWE4

bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6

-

Colletotrichum gloeosporioides KACC 40961

A0A0S2SWE4

i.e. Colletotrichum gloeosporioides

-

recombinant His-tagged wild-type and mutant enzymes 8fold from Escherichia coli strain ER2566 by nickel affinity chromatography

Colletotrichum gloeosporioides

mycelium

-

Colletotrichum gloeosporioides

-

0.39

-

purified recombinant enzyme, whole enzyme activity, substrate palmitoleate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.48

-

purified recombinant enzyme, whole enzyme activity, substrate oleate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.75

-

purified recombinant enzyme, whole enzyme activity, substrate alpha-linolenate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.87

-

pH 6.5, 40°C

Colletotrichum gloeosporioides

0.87

-

purified recombinant enzyme, whole enzyme activity, substrate (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.17

-

purified recombinant enzyme, N-terminal domain activity, substrate arachidate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.18

-

purified recombinant enzyme, N-terminal domain activity, substrate dihomo-gamma-linolenate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.2

-

purified recombinant enzyme, N-terminal domain activity, substrate eicosadienoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.39

-

purified recombinant enzyme, N-terminal domain activity, substrate eicosenoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

alpha-linolenate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxy-9,12,15-octadecatrienoate

-

?

alpha-linolenate + O2

via (9Z,12Z,15Z)-8-hydroperoxyoctadeca-9,12,15-trienoic acid, best substrate with respect to whole enzyme activity

741712

Colletotrichum gloeosporioides

(9Z,12Z,15Z)-7,8-dihydroxyoctadeca-9,12,15-trienoic acid

-

?

arachidonate + O2

-

741712

Colletotrichum gloeosporioides

(5Z,8Z,11Z,14Z)-8-hydroxyeicosa-5,8,11,14-tetraenoic acid

-

?

arachidonic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyarachidonic acid

-

?

dihomo-gamma-linolenate + O2

-

741712

Colletotrichum gloeosporioides

(8Z,11Z,14Z)-8-hydroxyeicosa-8,11,14-trienoic acid

-

?

dihomo-gamma-linolenic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxydihomo-gamma-linolenic acid

-

?

eicosadienoate + O2

-

741712

Colletotrichum gloeosporioides

(11Z,14Z)-8-hydroxyeicosa-11,14-dienoic acid

-

?

eicosadienoic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyeicosadienoic acid

-

?

eicosenoate + O2

-

741712

Colletotrichum gloeosporioides

(11Z)-8-hydroxyeicosa-11-enoic acid

-

?

eicosenoic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyeicosenoic acid

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(7S,8S)-7,8-dihydroxy-(9Z,12Z)-octadecadienoic acid

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid

-

?

additional information

bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6

741712

Colletotrichum gloeosporioides

?

-

?

additional information

the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, oleic acid, and palmitoleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). Substrate specificity, overview. The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (9Z,12Z)-7S,8S-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5R,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase. No activity with conjugated linoleic acid, docosapentaenoic acid, and docosahexaenoic acid, and no isomerase activity and no whole enzyme acitivity, but dioxygenase activity with eicosenoic acid, eicosadienoic acid, dihomo-gamma-linolenic acid, and arachidonic acid

741712

Colletotrichum gloeosporioides

?

-

?

additional information

bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6

741712

Colletotrichum gloeosporioides KACC 40961

?

-

?

additional information

the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, oleic acid, and palmitoleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). Substrate specificity, overview. The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (9Z,12Z)-7S,8S-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5R,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase. No activity with conjugated linoleic acid, docosapentaenoic acid, and docosahexaenoic acid, and no isomerase activity and no whole enzyme acitivity, but dioxygenase activity with eicosenoic acid, eicosadienoic acid, dihomo-gamma-linolenic acid, and arachidonic acid

741712

Colletotrichum gloeosporioides KACC 40961

?

-

?

oleate + O2

-

741712

Colletotrichum gloeosporioides

(9Z)-8-hydroperoxyoctadec-9-enoate

-

?

oleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(9Z)-8-hydroperoxyoctadec-9-enoate

-

?

oleate + O2

via (9Z)-8-hydroperoxyoctadeca-9-enoic acid

741712

Colletotrichum gloeosporioides

(9Z)-7,8-dihydroxyoctadeca-9-enoic acid

-

?

palmitoleate + O2

-

741712

Colletotrichum gloeosporioides

(9Z)-8-hydroperoxyhexadec-9-enoate

-

?

palmitoleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(9Z)-8-hydroperoxyhexadec-9-enoate

-

?

palmitoleate + O2

via (9Z)-dihydroperoxyhexadeca-9-enoate

741712

Colletotrichum gloeosporioides

(9Z)-5,8-dihydroxyhexadeca-9-enoic acid

-

?

homotetramer

4 * 127000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 127413, sequence calculation

Colletotrichum gloeosporioides

More

the enzyme diol synthase is a fusion protein, consisting of an N-terminal dioxygenase domain and a C-terminal cytochrome P450/hydroperoxide isomerase domain

Colletotrichum gloeosporioides

trimer

3 * 127000, SDS-PAGE, 3 * 127413, calculated

Colletotrichum gloeosporioides

8,11-hydroperoxide isomerase

-

Colletotrichum gloeosporioides

20

-

maximum of 8-diogenase reaction

Colletotrichum gloeosporioides

20

-

N-terminal domain activity

Colletotrichum gloeosporioides

40

-

maximum of whole enzyme

Colletotrichum gloeosporioides

40

-

whole enzyme activity

Colletotrichum gloeosporioides

20

50

purified recombinant enzyme, 2 h, the activities of whole 7,8-LDS and 8-dioxygenase in the N-terminal domain decrease as temperature increases. The N-terminal domain demonstrates a greater thermal stability than the C-terminal domain. The whole enzyme activity presentes 72% of the initial activity at 40°C after 2 h, while 8-dioxygenase activity in the N-terminal domain is 90%

Colletotrichum gloeosporioides

4.86

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

5.62

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

6.58

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

10.5

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.2

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.2

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.7

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.7

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

18.2

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

18.2

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

21.2

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

21.2

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

22

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

22

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

24.7

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

24.7

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

25.1

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

25.2

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

35

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

35

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

additional information

-

the maximum activity for the conversion of linoleic acid to (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) by 8-dioxygenase in the N-terminal domain of 7,8-LDS is observed at pH 7.0 and 20°C, whereas that for the conversion of linoleic acid to (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by the whole enzyme is observed at pH 6.5 and 40°C

Colletotrichum gloeosporioides

6.5

-

maximum of bifunctional enzyme

Colletotrichum gloeosporioides

6.5

-

whole enzyme activity

Colletotrichum gloeosporioides

7

-

maximum of 8-diogenase reaction

Colletotrichum gloeosporioides

7

-

N-terminal domain activity

Colletotrichum gloeosporioides

DMSO

the whole enzyme activity is the highest using DMSO among the organic solvents tested, and its optimal concentration is 2.5% v/v

Colletotrichum gloeosporioides

expression in Escherichia coli

Colletotrichum gloeosporioides

phylogenetic tree, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain ER2566

Colletotrichum gloeosporioides

A891G

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

C1038S

site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE

Colletotrichum gloeosporioides

H1036A

site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE

Colletotrichum gloeosporioides

H1036A/C1038S

site-directed mutagenesis, the double mutant converts linoleic acid to only 8-HPODE

Colletotrichum gloeosporioides

additional information

comparison of substrate specificities of wild-type an d mutant enzymes, overview

Colletotrichum gloeosporioides

N895D

site-directed mutagenesis, the mutant produces a reduced amount of 8-HPODE, compared to wild-type, without the accumulation of DiHODE

Colletotrichum gloeosporioides

N895L

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

N895Q

site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme

Colletotrichum gloeosporioides

Q898E

site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product

Colletotrichum gloeosporioides

Q898L

site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product

Colletotrichum gloeosporioides

Y380F

site-directed mutagenesis, the mutant enzyme produces trace amount of 7,8-DiHODE without the accumulation of 8-HPODE when incubated with linoleic acid as substrate

Colletotrichum gloeosporioides

0.0193

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0193

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.022

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0264

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0264

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0293

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0293

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0305

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0305

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0319

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0319

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0324

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0324

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0333

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0358

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0358

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0368

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0369

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.0371

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.048

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

510000

-

gel filtration

Colletotrichum gloeosporioides

510000

-

recombinant enzyme, gel filtration

Colletotrichum gloeosporioides

linoleate + O2

Colletotrichum gloeosporioides

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

-

?

DMSO

maximum activity in presence of 2.5% DMSO

Colletotrichum gloeosporioides

recombinant His-tagged wild-type and mutant enzymes 8fold from Escherichia coli strain ER2566 by nickel affinity chromatography

Colletotrichum gloeosporioides

mycelium

-

Colletotrichum gloeosporioides

-

0.39

-

purified recombinant enzyme, whole enzyme activity, substrate palmitoleate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.48

-

purified recombinant enzyme, whole enzyme activity, substrate oleate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.75

-

purified recombinant enzyme, whole enzyme activity, substrate alpha-linolenate, pH 6.5, 40°C

Colletotrichum gloeosporioides

0.87

-

pH 6.5, 40°C

Colletotrichum gloeosporioides

0.87

-

purified recombinant enzyme, whole enzyme activity, substrate (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.17

-

purified recombinant enzyme, N-terminal domain activity, substrate arachidate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.18

-

purified recombinant enzyme, N-terminal domain activity, substrate dihomo-gamma-linolenate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.2

-

purified recombinant enzyme, N-terminal domain activity, substrate eicosadienoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

1.39

-

purified recombinant enzyme, N-terminal domain activity, substrate eicosenoate, pH 6.5, 40°C

Colletotrichum gloeosporioides

alpha-linolenate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxy-9,12,15-octadecatrienoate

-

?

alpha-linolenate + O2

via (9Z,12Z,15Z)-8-hydroperoxyoctadeca-9,12,15-trienoic acid, best substrate with respect to whole enzyme activity

741712

Colletotrichum gloeosporioides

(9Z,12Z,15Z)-7,8-dihydroxyoctadeca-9,12,15-trienoic acid

-

?

arachidonate + O2

-

741712

Colletotrichum gloeosporioides

(5Z,8Z,11Z,14Z)-8-hydroxyeicosa-5,8,11,14-tetraenoic acid

-

?

arachidonic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyarachidonic acid

-

?

dihomo-gamma-linolenate + O2

-

741712

Colletotrichum gloeosporioides

(8Z,11Z,14Z)-8-hydroxyeicosa-8,11,14-trienoic acid

-

?

dihomo-gamma-linolenic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxydihomo-gamma-linolenic acid

-

?

eicosadienoate + O2

-

741712

Colletotrichum gloeosporioides

(11Z,14Z)-8-hydroxyeicosa-11,14-dienoic acid

-

?

eicosadienoic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyeicosadienoic acid

-

?

eicosenoate + O2

-

741712

Colletotrichum gloeosporioides

(11Z)-8-hydroxyeicosa-11-enoic acid

-

?

eicosenoic acid + O2

-

741712

Colletotrichum gloeosporioides

8-hydroxyeicosenoic acid

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(7S,8S)-7,8-dihydroxy-(9Z,12Z)-octadecadienoic acid

-

?

linoleate + O2

-

741712

Colletotrichum gloeosporioides

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid

-

?

additional information

bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6

741712

Colletotrichum gloeosporioides

?

-

?

additional information

the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, oleic acid, and palmitoleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). Substrate specificity, overview. The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (9Z,12Z)-7S,8S-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5R,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase. No activity with conjugated linoleic acid, docosapentaenoic acid, and docosahexaenoic acid, and no isomerase activity and no whole enzyme acitivity, but dioxygenase activity with eicosenoic acid, eicosadienoic acid, dihomo-gamma-linolenic acid, and arachidonic acid

741712

Colletotrichum gloeosporioides

?

-

?

additional information

bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6

741712

Colletotrichum gloeosporioides KACC 40961

?

-

?

additional information

the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, oleic acid, and palmitoleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). Substrate specificity, overview. The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (9Z,12Z)-7S,8S-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5R,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase. No activity with conjugated linoleic acid, docosapentaenoic acid, and docosahexaenoic acid, and no isomerase activity and no whole enzyme acitivity, but dioxygenase activity with eicosenoic acid, eicosadienoic acid, dihomo-gamma-linolenic acid, and arachidonic acid

741712

Colletotrichum gloeosporioides KACC 40961

?

-

?

oleate + O2

-

741712

Colletotrichum gloeosporioides

(9Z)-8-hydroperoxyoctadec-9-enoate

-

?

oleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(9Z)-8-hydroperoxyoctadec-9-enoate

-

?

oleate + O2

via (9Z)-8-hydroperoxyoctadeca-9-enoic acid

741712

Colletotrichum gloeosporioides

(9Z)-7,8-dihydroxyoctadeca-9-enoic acid

-

?

palmitoleate + O2

-

741712

Colletotrichum gloeosporioides

(9Z)-8-hydroperoxyhexadec-9-enoate

-

?

palmitoleate + O2

-

741712

Colletotrichum gloeosporioides KACC 40961

(9Z)-8-hydroperoxyhexadec-9-enoate

-

?

palmitoleate + O2

via (9Z)-dihydroperoxyhexadeca-9-enoate

741712

Colletotrichum gloeosporioides

(9Z)-5,8-dihydroxyhexadeca-9-enoic acid

-

?

homotetramer

4 * 127000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 127413, sequence calculation

Colletotrichum gloeosporioides

More

the enzyme diol synthase is a fusion protein, consisting of an N-terminal dioxygenase domain and a C-terminal cytochrome P450/hydroperoxide isomerase domain

Colletotrichum gloeosporioides

trimer

3 * 127000, SDS-PAGE, 3 * 127413, calculated

Colletotrichum gloeosporioides

20

-

maximum of 8-diogenase reaction

Colletotrichum gloeosporioides

20

-

N-terminal domain activity

Colletotrichum gloeosporioides

40

-

maximum of whole enzyme

Colletotrichum gloeosporioides

40

-

whole enzyme activity

Colletotrichum gloeosporioides

20

50

purified recombinant enzyme, 2 h, the activities of whole 7,8-LDS and 8-dioxygenase in the N-terminal domain decrease as temperature increases. The N-terminal domain demonstrates a greater thermal stability than the C-terminal domain. The whole enzyme activity presentes 72% of the initial activity at 40°C after 2 h, while 8-dioxygenase activity in the N-terminal domain is 90%

Colletotrichum gloeosporioides

4.86

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

5.62

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

6.58

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

10.5

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.2

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.2

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.7

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

17.7

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

18.2

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

18.2

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

21.2

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

21.2

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

22

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

22

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

24.7

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

24.7

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

25.1

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

25.2

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

35

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

35

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

additional information

-

the maximum activity for the conversion of linoleic acid to (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) by 8-dioxygenase in the N-terminal domain of 7,8-LDS is observed at pH 7.0 and 20°C, whereas that for the conversion of linoleic acid to (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by the whole enzyme is observed at pH 6.5 and 40°C

Colletotrichum gloeosporioides

6.5

-

maximum of bifunctional enzyme

Colletotrichum gloeosporioides

6.5

-

whole enzyme activity

Colletotrichum gloeosporioides

7

-

maximum of 8-diogenase reaction

Colletotrichum gloeosporioides

7

-

N-terminal domain activity

Colletotrichum gloeosporioides

100

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

170

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

340

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

370

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

466.1

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

470

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

508.4

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

510

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

554.9

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

560

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

679

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

680

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

690

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

695.1

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

840

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

843

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

950

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

954.54

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

1813.5

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

1820

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

100

-

palmitoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

170

-

oleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

340

-

alpha-linolenate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

370

-

linoleate

bifunctional enzyme, pH 6.5, 40°C

Colletotrichum gloeosporioides

466.1

-

dihomo-gamma-linolenate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

470

-

dihomo-gamma-linolenic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

508.4

-

arachidonate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

510

-

arachidonic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

554.9

-

eicosadienoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

560

-

eicosadienoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

679

-

eicosenoate

recombinant enzyme, N-terminal domain activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

680

-

eicosenoic acid

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

690

-

palmitoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

695.1

-

palmitoleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

840

-

oleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

843

-

oleate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

950

-

alpha-linolenate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

954.54

-

alpha-linolenate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

1813.5

-

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

recombinant enzyme, whole enzyme activity, pH 6.5, 40°C

Colletotrichum gloeosporioides

1820

-

linoleate

N-terminal 8-dioxygenase domain, pH 6.5, 40°C

Colletotrichum gloeosporioides

741712

Seo

Characterization of a recombi ...

Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961

Appl. Microbiol. Biotechnol.

100

3087-3099

2016

1

-

2

-

11

-

20

-

2

1

7

-

1

-

1

9

-

25

3

1

4

-

1

20

5

-

1

-

2

-

11

-

20

-

2

1

-

1

-

1

9

-

25

3

4

-

1

20

5

-

20

742730

Seo

Production of 7,8-dihydroxy u ...

Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961

J. Agric. Food Chem.

64

8555-8562

2016

1

2

-

1

-

2

-

11

-

1

-

1

-

14

-

3

1

-

1

-

1

2

-

1

-

2

-

1

-

1

-

14

-

1

-

1

-

743200

Sooman

Discovery of a novel linoleat ...

Colletotrichum graminicola, Fusarium oxysporum, Colletotrichum graminicola M1.001, Colletotrichum graminicola M1.001 / M2 / FGSC 10212, Fusarium oxysporum Fo5176

Lipids

50

1243-1252

2015

-

1

-

5

-

18

-

29

-

8

2

-

1

-

5

-

29

-

2

-

2

-

724132

Hoffmann

7,8- and 5,8-linoleate diol sy ...

Aspergillus fumigatus, Gaeumannomyces graminis

Arch. Biochem. Biophys.

539

87-91

2013

-

2

-

7

-

6

-

5

2

4

-

2

-

7

-

5

2

-

2

-

711024

Hoffmann

Expression of 5,8-LDS of Asper ...

Aspergillus fumigatus, Gaeumannomyces graminis

Arch. Biochem. Biophys.

506

216-222

2010

-

2

-

7

-

2

-

3

-

6

-

2

-

10

-

10

-

2

-

7

-

2

-

3

-

2

-

10

-

2

-

714317

Jernerén

Reaction mechanism of 5,8-lino ...

Aspergillus clavatus

Biochim. Biophys. Acta

1801

503-507

2010

-

1

-

7

-

2

-

3

-

1

-

2

-

704466

Brodhun

Identification of PpoA from As ...

Aspergillus nidulans

J. Biol. Chem.

284

11792-11805

2009

-

1

-

2

-

1

4

-

2

1

-

1

-

1

-

1

-

7

1

-

4

1

-

2

-

1

2

-

2

-

1

-

4

-

2

1

-

1

-

1

-

7

1

-

4

1

-

4

704475

Garscha

Leucine/valine residues direct ...

Gaeumannomyces graminis

J. Biol. Chem.

284

13755-13765

2009

-

1

-

4

-

1

-

2

-

2

-

2

-

1

-

4

-

1

-

2

-

702018

Garscha

Pichia expression and mutagene ...

Gaeumannomyces graminis, Gaeumannomyces graminis var. avenae

Biochem. Biophys. Res. Commun.

373

579-583

2008

-

1

-

3

-

2

-

6

-

1

-

4

-

2

-

1

-

3

-

2

-

1

-

4

-

703700

Garscha

Critical amino acids for the 8 ...

Gaeumannomyces graminis

FEBS Lett.

582

3547-3551

2008

-

1

-

11

-

1

-

1

-

3

-

1

-

1

-

11

-

1

-

285246

Hörnsten

Cloning of linoleate diol synt ...

Gaeumannomyces graminis

J. Biol. Chem.

274

28219-28224

1999

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

285251

Oliw

Catalytic properties of linole ...

Gaeumannomyces graminis

Adv. Exp. Med. Biol.

469

679-685

1999

2

-

2

-

1

-

3

-

1

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

285244

Su

A protein radical and ferryl i ...

Gaeumannomyces graminis

J. Biol. Chem.

273

20744-20751

1998

-

1

-

2

-

5

-

2

-

2

-

5

-

2

-

2

-

2

-

2

-

2

-

2

-

5

-

285243

Su

Purification and characterizat ...

Gaeumannomyces graminis

J. Biol. Chem.

271

14112-14118

1996

-

2

-

2

1

-

1

-

1

2

1

-

1

-

1

-

1

-

1

-

2

-

2

-

2

-

2

1

-

1

-

1

2

1

-

1

-

1

-

285249

Su

Studies on linoleic acid 8R-di ...

Gaeumannomyces graminis

Lipids

30

43-50

1995

-

1

16

-

1

-

6

-

1

-

1

-

1

-

1

-

1

-

1

-

16

-

1

-

1

-

1

-

1

-

1

-

285247

Brodowsky

BW A4C and other hydroxamic ac ...

Gaeumannomyces graminis

Eur. J. Pharmacol.

254

43-47

1994

-

12

-

1

-

4

-

1

-

1

-

1

-

1

-

6

-

6

12

-

1

-

1

-

1

-

1

-

1

-

285248

Brodowsky

Linoleic acid 8R-dioxygenase a ...

Gaeumannomyces graminis

Ann. N. Y. Acad. Sci.

744

314-316

1994

-

5

-

1

-

3

-

1

-

2

-

5

-

5

-

1

-

1

-

285250

Hamberg

Sequential oxygenation of lino ...

Gaeumannomyces graminis

Arch. Biochem. Biophys.

309

77-80

1994

-

2

-

1

-

4

-

2

-

4

-

285245

Brodowsky

A linoleic acid (8R)-dioxygena ...

Gaeumannomyces graminis

J. Biol. Chem.

267

14738-14745

1992

-

1

-

1

-

1

-

1

-

1

-

Characterization of a recombinant 7,8-linoleate diol synthase from Glomerella cingulate

Data extracted from this reference: