EC Number   |
Protein Variants |
Reference  |
|---|
   1.13.11.60 | N895L |
site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme |
-, 741712 |
| 1.13.11.60 | N895Q |
site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme |
741712 |
| 1.13.11.60 | Q898E |
site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product |
741712 |
| 1.13.11.60 | Q898L |
site-directed mutagenesis, the mutant converts linoleic acid to 8,11-DiHODE as major product and 7,8-DiHODE as minor product |
741712 |
| 1.13.11.60 | Y380F |
site-directed mutagenesis, the mutant enzyme produces trace amount of 7,8-DiHODE without the accumulation of 8-HPODE when incubated with linoleic acid as substrate |
-, 741712 |
| 1.13.11.60 | more |
reaction conditions for the production of (7S,8S,9Z,12Z)-dihydroxyoctadeca-9,12-dienoic acid from linoleic acid by recombinant Escherichia coli expressing 7,8-linoleate diol synthase from Glomerella cingulata are optimized using response surface methodology. The optimal reaction conditions are pH 7.0, 18.6°C, 10.8% v/v dimethyl sulfoxide, 44.9 g/l cells, and 14.3 g/l linoleic acid, with agitation at 256 rpm. Under these conditions, recombinant cells produce 7,8-dihydroxy-unsaturated fatty acids in the range of 7.0-9.8 g/l from 14.3 g/l linoleic acid, 14.3 g/l oleic acid, and plant oil hydrolysates such as waste oil and olive oil containing 14.3 g/l linoleic acid or oleic acid. Comparisons of quantitative production of dihydroxy unsaturated fatty acids by microorganisms |
-, 742730 |
| 1.13.11.60 | C1005S |
the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation |
764229 |
| 1.13.11.60 | D749A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | E728A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | F794E |
the mutant acts as 7,8-linoleate diol synthase |
-, 764229 |
