EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.60 | Y374 |
the mutant enzyme shows no detectable activity when incubated with (9Z,12Z)-octadeca-9,12-dienoate as a substrate. When incubated with the intermediate product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate it is able to catalyze isomerization to (5S,8R,9Z,12Z)-5,8-dihydroperoxy-9,12-octadecadienoate |
704466 |
| 1.13.11.60 | C1006S/V328F |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | C1006S/V328L |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | C1006S/V328S |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | D749A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | E728A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | F794Y |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | F798Y |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | H1003A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | Q887A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
