EC Number |
Cofactor |
Reference |
---|
2.8.1.4 | ATP |
- |
643806, 643807, 643808, 643809, 643810, 643812 |
2.8.1.4 | ATP |
absolute requirement, other nucleotides are inactive |
643805 |
2.8.1.4 | more |
beta-mercaptopyruvate is inactive either as a cofactor or as a sulfur donor |
643806 |
2.8.1.4 | more |
sulfur transfer obtained with ADP is apparently due to the formation of ATP by the reaction 2 ADP = ATP + AMP, AMP is totally inactive as activating nucleotide |
643808 |
2.8.1.4 | more |
the enzyme requires two conserved Cys residues (Cys265 and Cys268), which are arranged in a CXXC motif in the PP-loop domain, for both in vitro and in vivo activities to generate 4-thiouridine in tRNAs |
725494 |
2.8.1.4 | pyridoxal 5'-phosphate |
- |
643809 |