EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.2.1.81 | -999 |
- |
mutant L122Q/N446I has higher thermostability than wild-type AgaB. Melting temperature of S2 is 4.6°C higher than that of wild-type AgaB, and the half-life of mutant L122Q/N446I is 350 min at 40°C, which is 18.4fold longer than that of the wild-type enzyme. Mutant N446I has similar enhanced thermostability as mutant L122Q/N446I. L122Q shows similar thermostability with wild-type AgaB. Half-life of mutant N446V at 40°C is 18.2fold longer than that of wild-type AgaB. Half-life of mutant N446L at 40°C is 12.9fold longer than that of wild-type AgaB |
695671 |
| 3.2.1.81 | 4 |
30 |
the enzyme retains its activity when the temperature was between 4 and 30°C for 1 h |
702825 |
| 3.2.1.81 | 10 |
50 |
up to 80% thermostability of the enzyme is retained at 10°C and 30°C for 180 min. However, when the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. The enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min |
750214 |
| 3.2.1.81 | 20 |
40 |
the enzyme is stable from 20 to 40°C after 30 min of incubatiopn. At 40°C, the enzyme retains 90% activity, however, it drastically decreases by 22% of the original value at 42.5°C |
732320 |
| 3.2.1.81 | 20 |
45 |
heat treatment in the range of 20-40°C does not affect the residual activity of the enzyme. Only 45% of the activity is retained after heating at 50°C for 1 h |
713923 |
| 3.2.1.81 | 25 |
- |
stable up to 25°C |
705484 |
| 3.2.1.81 | 25 |
45 |
the enzyme activity is stable at 25°C and 35°C. The enzyme retains more than 80% activity after 2 h exposure at the indicated temperature. However, it maintains less than 40% activity after 1 h at 45°C and 55°C |
732321 |
| 3.2.1.81 | 25 |
45 |
the enzyme is stable at temperatures below 45°C. The thermostability of the enzyme begins to decrease precipitously above 50°C, while less than 10% of maximum activity remain at 70°C |
752104 |
| 3.2.1.81 | 30 |
- |
inhibition above 30°C, after 20 min 35°C 50% of activity |
136886 |
| 3.2.1.81 | 30 |
- |
stable up to 30°C |
705484 |
