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Literature summary for 3.2.1.81 extracted from

  • Li, J.; Hu, Q.; Li, Y.; Xu, Y.
    Purification and characterization of cold-adapted beta-agarase from an antarctic psychrophilic strain (2015), Braz. J. Microbiol., 46, 683-690 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.81

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 90% residual activity at 2 mM Pseudoalteromonas sp. NJ21
Cd2+ about 85% residual activity at 2 mM Pseudoalteromonas sp. NJ21
Cu2+ complete inhibition at 2 mM Pseudoalteromonas sp. NJ21
EDTA about 20% residual activity at 2 mM Pseudoalteromonas sp. NJ21
Fe2+ about 70% residual activity at 2 mM Pseudoalteromonas sp. NJ21
additional information not inhibited by Cu2+ and Co2+ Pseudoalteromonas sp. NJ21

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudoalteromonas sp. NJ21
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ about 150% activity at 2 mM Pseudoalteromonas sp. NJ21
Mg2+ about 120% activity at 2 mM Pseudoalteromonas sp. NJ21
Mn2+ about 125% activity at 2 mM Pseudoalteromonas sp. NJ21

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agar + H2O Pseudoalteromonas sp. NJ21
-
 neoagarobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. NJ21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-200 gel filtration Pseudoalteromonas sp. NJ21

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
373.62
-
 47.78fold purified enzyme, at pH 8.0 and 40°C Pseudoalteromonas sp. NJ21

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Pseudoalteromonas sp. NJ21 neoagarobiose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Pseudoalteromonas sp. NJ21

Synonyms

Synonyms Comment Organism
Aga21
-
 Pseudoalteromonas sp. NJ21

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudoalteromonas sp. NJ21

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 the enzyme maintains 85% of the maximum activity at 10°C, the enzyme activity drops at temperatures higher than 40°C Pseudoalteromonas sp. NJ21

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 up to 80% thermostability of the enzyme is retained at 10°C and 30°C for 180 min. However, when the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. The enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min Pseudoalteromonas sp. NJ21

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudoalteromonas sp. NJ21

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 the enzyme is stable (more than 70% relative activity) in buffers with a pH range of 6.5-8.5 Pseudoalteromonas sp. NJ21