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Literature summary for 3.2.1.81 extracted from

  • Ariga, O.; Inoue, T.; Kubo, H.; Minami, K.; Nakamura, M.; Iwai, M.; Moriyama, H.; Yanagisawa, M.; Nakasaki, K.
    Cloning of agarase gene from non-marine agarolytic bacterium Cellvibrio sp. (2012), J. Microbiol. Biotechnol., 22, 1237-1244.
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Cellvibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78771
-
 1 * 78771, calculated from amino acid sequence Cellvibrio sp.
79000
-
 gel filtration Cellvibrio sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Cellvibrio sp.
-
 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?
agarose + H2O Cellvibrio sp. OA-2007
-
 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?

Organism

Organism UniProt Comment Textmining
Cellvibrio sp. B3IXL6
-
-
Cellvibrio sp. OA-2007 B3IXL6
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Toyopearl PAK-650M column chromatography, and Sephacryl S-200 gel filtration Cellvibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Cellvibrio sp. neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?
agarose + H2O
-
 Cellvibrio sp. OA-2007 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 79000, SDS-PAGE Cellvibrio sp.
monomer 1 * 78771, calculated from amino acid sequence Cellvibrio sp.

Synonyms

Synonyms Comment Organism
AgaA
-
 Cellvibrio sp.
agarase
-
 Cellvibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42.5
-
-
 Cellvibrio sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 47 more than 50% activity between 25 and 47°C. The enzyme activity drastically decreases over 45°C Cellvibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme is stable from 20 to 40°C after 30 min of incubatiopn. At 40°C, the enzyme retains 90% activity, however, it drastically decreases by 22% of the original value at 42.5°C Cellvibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Cellvibrio sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 50% activity between pH 6.0 and 8.0. The activity gradually decreases at alkaline pH and significantly declines at acidic pH Cellvibrio sp.