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Literature summary for 3.2.1.81 extracted from
- Agarase: review of major sources, categories, purification method, enzyme characteristics and applications (2010), Mar. drugs, 8, 200-218.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Bacillus subtilis | Microbulbifer thermotolerans |
| expressed in Escherichia coli BL21 cells | Pseudomonas sp. |
| expressed in Escherichia coli BL21 cells | Vibrio sp. |
| expressed in Escherichia coli BL21 cells | Agarivorans sp. |
| expressed in Escherichia coli BL21 cells | Vibrio sp. PO-303 |
| expressed in Escherichia coli DH5alpha cells | Vibrio sp. |
| expressed in Escherichia coli DH5alpha cells | Zobellia galactanivorans |
| expressed in Escherichia coli DH5alpha cells | Agarivorans albus |
| expressed in Escherichia coli DH5alpha cells | Agarivorans sp. |
| expressed in Escherichia coli EPI300 cells | Saccharophagus degradans 2-40 |
| expressed in Escherichia coli EPI300 cells | Saccharophagus degradans |
| expressed in Escherichia coli JM83 cells | Pseudomonas sp. |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Vibrio sp. | - |
- |
| extracellular | - |
Cytophaga sp. | - |
- |
| extracellular | - |
Flavobacterium flevense | - |
- |
| extracellular | - |
Pseudoalteromonas antarctica | - |
- |
| extracellular | - |
Alteromonas sp. | - |
- |
| extracellular | - |
Agarivorans sp. | - |
- |
| extracellular | - |
Agarivorans albus | - |
- |
| extracellular | - |
Bacillus sp. MK03 | - |
- |
| extracellular | - |
Pseudomonas sp. | - |
- |
| extracellular | - |
Microbulbifer thermotolerans | - |
- |
| extracellular | - |
Vibrio sp. PO-303 | - |
- |
| intracellular | - |
Pseudomonas sp. | 5622 | - |
| intracellular | - |
Zobellia galactanivorans | 5622 | - |
| intracellular | - |
Agarivorans sp. | 5622 | - |
| intracellular | - |
Saccharophagus degradans 2-40 | 5622 | - |
| intracellular | - |
Saccharophagus degradans | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
- |
Flavobacterium flevense |
| 31000 | - |
recombinant enzyme | Zobellia galactanivorans |
| 33000 | - |
- |
Pseudoalteromonas antarctica |
| 37000 | - |
recombinant enzyme | Pseudomonas sp. |
| 39500 | - |
- |
Alteromonas sp. |
| 48000 | - |
recombinant enzyme | Microbulbifer thermotolerans |
| 49000 | - |
recombinant enzyme | Microbulbifer thermotolerans |
| 50000 | - |
- |
Agarivorans albus |
| 51000 | - |
- |
Agarivorans sp. |
| 51000 | - |
recombinant enzyme | Vibrio sp. PO-303 |
| 52000 | - |
- |
Alteromonas sp. |
| 52000 | - |
- |
Vibrio sp. |
| 58000 | - |
- |
Agarivorans sp. |
| 59000 | - |
recombinant enzyme | Pseudomonas sp. |
| 60000 | - |
recombinant enzyme | Zobellia galactanivorans |
| 64000 | - |
recombinant enzyme | Saccharophagus degradans |
| 86000 | - |
recombinant enzyme | Saccharophagus degradans 2-40 |
| 87000 | - |
recombinant enzyme | Saccharophagus degradans 2-40 |
| 87500 | - |
native enzyme | Vibrio sp. PO-303 |
| 89000 | - |
recombinant enzyme | Saccharophagus degradans |
| 92000 | - |
x * 92000, SDS-PAGE | Bacillus sp. MK03 |
| 107000 | - |
- |
Vibrio sp. |
| 109000 | - |
recombinant enzyme | Agarivorans sp. |
| 113000 | - |
gel filtration | Bacillus sp. MK03 |
| 146000 | - |
recombinant enzyme | Saccharophagus degradans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans albus | A8W969 | - |
- |
| Agarivorans albus YKW-34 | A8W969 | - |
- |
| Agarivorans sp. | - |
- |
- |
| Agarivorans sp. | A1E2A6 | - |
- |
| Agarivorans sp. | C9WIW7 | - |
- |
| Agarivorans sp. JA-1 | A1E2A6 | - |
- |
| Alteromonas sp. | - |
- |
- |
| Alteromonas sp. C-1 | - |
- |
- |
| Alteromonas sp. SY37-12 | - |
- |
- |
| Bacillus sp. MK03 | - |
- |
- |
| Cytophaga sp. | - |
- |
- |
| Flavobacterium flevense | - |
- |
- |
| Microbulbifer thermotolerans | Q6F4N4 | - |
- |
| Microbulbifer thermotolerans JAMB-A94 | Q6F4N4 | - |
- |
| Pseudoalteromonas antarctica | - |
- |
- |
| Pseudoalteromonas antarctica N-1 | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. | Q9KK55 | - |
- |
| Pseudomonas sp. SK38 | - |
- |
- |
| Pseudomonas sp. W7 | Q9KK55 | - |
- |
| Saccharophagus degradans | Q21HB4 | - |
- |
| Saccharophagus degradans | Q21HC5 | - |
- |
| Saccharophagus degradans | Q21LJ2 | - |
- |
| Saccharophagus degradans 2-40 | Q21HB9 | - |
- |
| Saccharophagus degradans 2-40 | Q21LJ1 | - |
- |
| Saccharophagus degradans 2-40 / ATCC 43961 | Q21HB4 | - |
- |
| Saccharophagus degradans 2-40 / ATCC 43961 | Q21HC5 | - |
- |
| Saccharophagus degradans 2-40 / ATCC 43961 | Q21LJ2 | - |
- |
| Vibrio sp. | - |
- |
- |
| Vibrio sp. | A1E2B6 | - |
- |
| Vibrio sp. JT0107 | - |
- |
- |
| Vibrio sp. PO-303 | A5A6R7 | - |
- |
| Vibrio sp. V134 | A1E2B6 | - |
- |
| Zobellia galactanivorans | G0L322 | - |
- |
| Zobellia galactanivorans | Q9RGX8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Alteromonas sp. | neoagarotetraose | - |
? |  |
| agarose + H2O | - |
Pseudomonas sp. | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Microbulbifer thermotolerans | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Alteromonas sp. C-1 | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Pseudomonas sp. W7 | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Microbulbifer thermotolerans JAMB-A94 | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose | - |
? | |
| agarose + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| agarose + H2O | - |
Cytophaga sp. | ? | - |
? | |
| agarose + H2O | - |
Agarivorans sp. | ? | - |
? | |
| agarose + H2O | - |
Saccharophagus degradans 2-40 | ? | - |
? | |
| agarose + H2O | - |
Saccharophagus degradans | ? | - |
? | |
| agarose + H2O | - |
Pseudomonas sp. SK38 | ? | - |
? | |
| agarose + H2O | - |
Saccharophagus degradans 2-40 / ATCC 43961 | ? | - |
? | |
| agarose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Flavobacterium flevense | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Agarivorans albus | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Bacillus sp. MK03 | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Agarivorans sp. | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Vibrio sp. JT0107 | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Agarivorans sp. JA-1 | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Agarivorans albus YKW-34 | neoagarotetraose + neoagarobiose | - |
? | |
| agarose + H2O | - |
Pseudoalteromonas antarctica | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Zobellia galactanivorans | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Agarivorans sp. | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Vibrio sp. PO-303 | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Pseudoalteromonas antarctica N-1 | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Vibrio sp. V134 | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Alteromonas sp. | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? | |
| agarose + H2O | - |
Alteromonas sp. C-1 | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? | |
| agarose + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 92000, SDS-PAGE | Bacillus sp. MK03 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-agarase A | - |
Zobellia galactanivorans |
| beta-agarase B | - |
Zobellia galactanivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | recombinant enzyme | Pseudomonas sp. |
| 30 | - |
- |
Pseudomonas sp. |
| 30 | - |
- |
Vibrio sp. |
| 30 | - |
- |
Alteromonas sp. |
| 30 | - |
recombinant enzyme | Agarivorans albus |
| 35 | - |
- |
Flavobacterium flevense |
| 35 | - |
- |
Alteromonas sp. |
| 38 | 55 | native enzyme | Vibrio sp. PO-303 |
| 40 | - |
- |
Cytophaga sp. |
| 40 | - |
- |
Bacillus sp. MK03 |
| 40 | - |
- |
Vibrio sp. |
| 40 | - |
- |
Agarivorans sp. |
| 40 | - |
recombinant enzyme | Agarivorans sp. |
| 40 | - |
recombinant enzyme | Vibrio sp. PO-303 |
| 40 | - |
native enzyme | Agarivorans albus |
| 50 | - |
- |
Microbulbifer thermotolerans |
| 55 | - |
- |
Microbulbifer thermotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
stable up to 25°C | Agarivorans sp. |
| 30 | - |
stable up to 30°C | Pseudoalteromonas antarctica |
| 30 | - |
stable up to 30°C | Alteromonas sp. |
| 30 | - |
stable up to 40°C | Vibrio sp. |
| 35 | - |
stable up to 35°C | Bacillus sp. MK03 |
| 37 | - |
stable up to 37°C | Pseudomonas sp. |
| 40 | - |
stable up to 40°C | Flavobacterium flevense |
| 45 | - |
stable up to 45°C | Vibrio sp. PO-303 |
| 50 | - |
stable up to 50°C | Alteromonas sp. |
| 50 | - |
stable up to 50°C | Agarivorans albus |
| 50 | - |
stable up to 50°C | Microbulbifer thermotolerans |
| 50 | - |
stable up to 50°C | Agarivorans sp. |
| 60 | - |
stable up to 60°C | Microbulbifer thermotolerans |
| 60 | - |
70% activity at 60°C | Agarivorans sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Agarivorans sp. |
| 6.3 | - |
- |
Flavobacterium flevense |
| 6.5 | - |
- |
Alteromonas sp. |
| 6.5 | 7.5 | native enzyme | Vibrio sp. PO-303 |
| 7 | - |
- |
Pseudoalteromonas antarctica |
| 7 | - |
- |
Alteromonas sp. |
| 7 | - |
- |
Microbulbifer thermotolerans |
| 7 | - |
- |
Vibrio sp. |
| 7 | - |
- |
Agarivorans sp. |
| 7 | - |
recombinant enzyme | Agarivorans albus |
| 7.2 | - |
- |
Cytophaga sp. |
| 7.5 | - |
recombinant enzyme | Vibrio sp. PO-303 |
| 7.6 | - |
- |
Bacillus sp. MK03 |
| 7.8 | - |
- |
Pseudomonas sp. |
| 8 | - |
- |
Vibrio sp. |
| 8 | - |
- |
Agarivorans sp. |
| 8 | - |
native enzyme | Agarivorans albus |
| 9 | - |
- |
Pseudomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | - |
Agarivorans sp. |
| 4 | 8 | - |
Vibrio sp. PO-303 |
| 5 | 8 | - |
Microbulbifer thermotolerans |
| 6 | 9 | - |
Flavobacterium flevense |
| 6 | 9 | - |
Agarivorans albus |
| 6 | 9 | - |
Microbulbifer thermotolerans |
| 7.1 | 8.2 | - |
Bacillus sp. MK03 |
| 7.1 | 8.2 | - |
Pseudomonas sp. |
| 7.1 | 8.2 | - |
Agarivorans sp. |
| 8 | 9 | - |
Pseudomonas sp. |
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