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Literature summary for 3.2.1.81 extracted from
- Cloning, expression, and characterization of a glycoside hydrolase family 118 beta-agarase from Agarivorans sp. JA-1 (2012), J. Microbiol. Biotechnol., 22, 1692-1697.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Agarivorans sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Agarivorans sp. |
| 52580 | - |
x * 52580, calculated from amino acid sequence | Agarivorans sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Agarivorans sp. | - |
neoagarooctaose + ? | main product (26.7% of total products) | ? |  |
| agarose + H2O | Agarivorans sp. JA-1 | - |
neoagarooctaose + ? | main product (26.7% of total products) | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans sp. | K7WTF8 | - |
- |
| Agarivorans sp. JA-1 | K7WTF8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chitin bead column chromatography | Agarivorans sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
cell-free extract, at pH 7.0 and 35°C | Agarivorans sp. |
| 208.1 | - |
purified enzyme, at pH 7.0 and 35°C | Agarivorans sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Agarivorans sp. | neoagarooctaose + ? | main product (26.7% of total products) | ? | |
| agarose + H2O | - |
Agarivorans sp. JA-1 | neoagarooctaose + ? | main product (26.7% of total products) | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Agarivorans sp. |
| ? | x * 52580, calculated from amino acid sequence | Agarivorans sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agarase | - |
Agarivorans sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Agarivorans sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | the enzyme activity is more than 80% at 25°C and 45°C and less than 10% over 55°C | Agarivorans sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | the enzyme activity is stable at 25°C and 35°C. The enzyme retains more than 80% activity after 2 h exposure at the indicated temperature. However, it maintains less than 40% activity after 1 h at 45°C and 55°C | Agarivorans sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Agarivorans sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the enzyme activity is about 80% at pH 6.0 and less than 60% over pH 8.0 | Agarivorans sp. |
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