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EC Number	Temperature Stability Minimum [�C]	Temperature Stability Maximum [�C]	Commentary	Reference
2.4.1.20	37	60	in contrast to the rapid inactivation at 60°C, the enzyme is remarkably stable at 37°C. At 50°C, the enzyme retains over 58% of its initial activity after 24 h	735417
2.4.1.20	40	-	inactivated above	637858, 637859
2.4.1.20	40	-	stable up to	719128
2.4.1.20	40	-	the enzyme is stable below 40°C	735893
2.4.1.20	50	-	unstable above	637863
2.4.1.20	60	-	complete inactivation within 10 min	637858, 637859
2.4.1.20	60	-	stable for at least 42 h	489244
2.4.1.20	70	-	half-life of wild-type 8.3 min, mutant Q130H/K131Y/S411G/A423S/A781K 17.7 min, mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K 24.6 min	720434
2.4.1.20	70	-	less than 10% loss of activity after heating for 30 min at pH 6-8	637868
2.4.1.20	75	-	the enzyme retains 80% of its initial activity after incubating at 75°C and pH 7.5 for 30 min	758413

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Release 2023.1 (January 2023)