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Information on EC 2.4.1.20 - cellobiose phosphorylase
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2.4.1.20 cellobiose phosphorylaseSpecify your search results
Word Map
- 2.4.1.20
-
phosphorolysis
- cellodextrins
- phosphorylases
-
cellvibrio
- gilvus
-
cellulolytic
- thermocellum
-
cellulomonas
- synthesis
- alpha-d-glucose
- laminaribiose
-
cellobiose-grown
- cellotriose
- alpha-d-glucose-1-phosphate
-
phosphorolyzed
- 6-deoxy-d-glucose
- analysis
The enzyme appears in viruses and cellular organisms
Synonyms
cellobiose phosphorylase, lactose phosphorylase, gh94 cellobiose phosphorylase, cellobiose phosphorylase a, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Cbp
CbpA
cellobiose phosphorylase A
cellobiose:orthophosphate alpha-D-glucosyltransferase
-
-
-
-
CPase
ra2122
THA_1941
Tm_1848
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
computational analyses to elucidate the conformational itinerary along the reaction pathway of this enzyme using the substrate binding structure determined from crystal structure, predicted reaction pathway, overview
-
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
reaction proceeds through a random-ordered bi-bi mechanism, by which phosphate and cellobiose bind in random order and D-glucose is released before glucose 1-phosphate
-
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
reaction proceeds through a random-ordered bi-bi mechanism, by which phosphate and cellobiose bind in random order and D-glucose is released before glucose 1-phosphate
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
cellobiose:phosphate alpha-D-glucosyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
9030-20-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D -glucose 1-fluoride + D-glucose
cellobiose + fluoride
-
-
-
-
ir
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + 5a-carba-alpha-D,L-glucopyranose
4-O-beta-D-glucopyranosyl-5a-carba-beta-D-glucopyranose + phosphate
-
very poor substrate
-
-
?
alpha-D-glucose 1-phosphate + 5a-carba-beta-D,L-glucopyranose
?
-
much better substrate than alpha-anomer, 22% of activity with D-glucose
-
-
?
alpha-D-glucose 1-phosphate + butyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
? + phosphate
-
no substrate for wilalpha-D-type
-
-
r
alpha-D-glucose 1-phosphate + D-glucosamine
4-O-beta-D-glucopyranosyl-D-glucosamine
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
4-O-beta-D-glucopyranosyl-D-mannose
-
-
-
-
?
alpha-D-glucose 1-phosphate + ethyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + methyl alpha-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
4-O-beta-D-glucopyranosyl-N-acetyl-D-glucosamine
-
substrate for mutant Y648V only
-
-
?
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
beta-D-glucose 1-phosphate + alpha-D-glucopyranosyl fluoride
beta-cellobiose + HF
-
beta-D-glucopyranosyl fluoride is not ulilized
-
?
cellobiose + arsenate
D-glucose + alpha-D-glucose 1-arsenate
-
-
alpha-D-glucose 1-arsenate hydrolyses spontaneously to form D-glucose and arsenate
ir
cellopentaose + phosphate
?
63.3% activity compared to cellotetraose
-
-
r
cellotriose + phosphate
?
89.7% activity compared to cellotetraose
-
-
r
D-glucal + 2-deoxy-2-(hydroxymethyl)-beta-D-xylopyranose
2-deoxy-3-O-(2-deoxy-beta-D-arabino-hexopyranosyl)-2-(hydroxymethyl)-beta-D-xylopyranose + ?
-
-
-
-
r
D-glucal + D-glucose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-glucose + ?
-
-
-
-
r
D-glucal + D-mannose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-mannose + ?
-
-
-
-
r
D-glucal + D-xylose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-xylose + ?
-
-
-
-
r
D-glucal + phosphate
2-deoxy-alpha-D-glucose 1-phosphate
-
D-glucal is a slow alternative donor substrate for stereospecific glycosyl transfer to phosphate. Reaction follows a stereochemical course where substrate becomes protonated from below its six-membered ring through stereoselective re side attack at C-2. The proposed catalytic mechanism involves direct protonation of D-glucal by the enzyme-bound phosphate, which then performs nucleophilic attack on the reactive C-1 of donor substrate. When offered D-glucose next to D-glucal and phosphate, the enzyme produces 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose and 2-deoxy-alpha-D-glucose 1-phosphate in a ratio governed by mass action of the two acceptor substrates present. Enzymatic synthesis of 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose is effectively promoted by catalytic concentrations of phosphate, suggesting that catalytic reaction proceeds through a quaternary complex of enzyme, D-glucal, phosphate,and D-glucose
-
-
?
lactose + phosphate
alpha-D-galactose 1-phosphate + D-glucose
-
-
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
much better substrate than 2-deoxy-D-glucose
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-arabinose
?
-
very poor substrate
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
in synthesis reaction, glucose is best substrate
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
in synthesis reaction, glucose is best substrate
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
-
no activity with L-isomer
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
no activity with L-isomer
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
higher specific activity than with D-glucose as acceptor
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
the enzyme shows beta-anomer selective glucosylation activity towards disaccharides
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
the enzyme shows beta-anomer selective glucosylation activity towards disaccharides
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-D-cellobiose is phosphorylyzed faster than alpha-anomer, ordered bi-bi mechanism
beta-D-glucose was shown to be the 4 times better acceptor compared to alpha-D-glucose in the reverse reaction
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
CBP catalyzes the phosphorolysis of cellobiose into alpha-D-glucose 1-phosphate and D-glucose with inversion of anomeric configuration, computational docking, overview
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ternary-complex kinetic mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
important for energy efficient catabolism of cellobiose in cytoplasm
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
38.6% activity compared to cellotetraose
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
random ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
random ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
O52504
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
O52504
nonconventional pathway for glucan utilization
-
-
?
additional information
?
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
-
no activity with D-xylose and D-mannose
-
-
?
additional information
?
-
-
no activity detected with glucose-acceptor different from D-glucose
-
-
?
additional information
?
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate
-
-
-
additional information
?
-
-
beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate
-
-
-
additional information
?
-
O52504
only disaccharide accepted as substrate
-
-
?
additional information
?
-
-
only disaccharide accepted as substrate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?