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Literature summary for 2.4.1.20 extracted from

  • Hamura, K.; Saburi, W.; Abe, S.; Morimoto, N.; Taguchi, H.; Mori, H.; Matsui, H.
    Enzymatic characteristics of cellobiose phosphorylase from Ruminococcus albus NE1 and kinetic mechanism of unusual substrate inhibition in reverse phosphorolysis (2012), Biosci. Biotechnol. Biochem., 76, 812-818.
    View publication on PubMed
Search for more literature for 2.4.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose inhibition of the synthetic reaction, binds competitively at the glucose 1-phosphate site and uncompetitvely at the ternary enzyme-glucose 1-phosphate-glucose complex Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.46
-
 D-glucose pH 6.0, 37°C Ruminococcus albus
9.5
-
 1,5-anhydro-D-glucitol pH 6.0, 37°C Ruminococcus albus
9.8
-
 6-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus
10.8
-
 D-gentobiose pH 6.0, 37°C Ruminococcus albus
13.3
-
 D-glucosamine pH 6.0, 37°C Ruminococcus albus
23.5
-
 D-mannose pH 6.0, 37°C Ruminococcus albus
25.5
-
 D-xylose pH 6.0, 37°C Ruminococcus albus
60.2
-
 2-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 2 * 94000, SDS-PAGE Ruminococcus albus
187000
-
 gel filtration Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
-
-
-
Ruminococcus albus NE1
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose reaction proceeds through a random-ordered bi-bi mechanism, by which phosphate and cellobiose bind in random order and D-glucose is released before glucose 1-phosphate Ruminococcus albus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
-
 Ruminococcus albus NE1 ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Ruminococcus albus NE1 ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Ruminococcus albus NE1 ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-gentobiose
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-glucosamine
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-glucose in synthesis reaction, glucose is best substrate Ruminococcus albus cellobiose + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-glucose in synthesis reaction, glucose is best substrate Ruminococcus albus NE1 cellobiose + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-mannose
-
 Ruminococcus albus ? + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-xylose
-
 Ruminococcus albus ? + phosphate
-
 r
cellobiose + phosphate
-
 Ruminococcus albus alpha-D-glucose 1-phosphate + D-glucose
-
 r
cellobiose + phosphate
-
 Ruminococcus albus NE1 alpha-D-glucose 1-phosphate + D-glucose
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 94000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Ruminococcus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 1,5-anhydro-D-glucitol pH 6.0, 37°C Ruminococcus albus
2.83
-
 D-mannose pH 6.0, 37°C Ruminococcus albus
4
-
 D-gentobiose pH 6.0, 37°C Ruminococcus albus
9.56
-
 D-glucosamine pH 6.0, 37°C Ruminococcus albus
16.5
-
 D-xylose pH 6.0, 37°C Ruminococcus albus
21.5
-
 2-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus
92.3
-
 D-glucose pH 6.0, 37°C Ruminococcus albus
124
-
 6-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Ruminococcus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.8 stable within Ruminococcus albus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
 D-mannose pH 6.0, 37°C Ruminococcus albus
0.29
-
 1,5-anhydro-D-glucitol pH 6.0, 37°C Ruminococcus albus
0.36
-
 2-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus
0.37
-
 D-gentobiose pH 6.0, 37°C Ruminococcus albus
0.65
-
 D-xylose pH 6.0, 37°C Ruminococcus albus
0.72
-
 D-glucosamine pH 6.0, 37°C Ruminococcus albus
12.7
-
 6-deoxy-D-glucose pH 6.0, 37°C Ruminococcus albus
63.2
-
 D-glucose pH 6.0, 37°C Ruminococcus albus