Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | strong inhibition at high concentrations | Acetivibrio thermocellus | |
N-ethylmaleimide | - |
Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | |
10 | - |
D-glucosamine | - |
Acetivibrio thermocellus | |
40 | - |
D-xylose | - |
Acetivibrio thermocellus | |
170 | - |
D-mannose | - |
Acetivibrio thermocellus | |
250 | - |
2-deoxy-D-glucose | - |
Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | not required | Acetivibrio thermocellus | |
additional information | no influence of metal ions on reaction | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
SDS-PAGE | Acetivibrio thermocellus |
150000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | Acetivibrio thermocellus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
strain ATCC 27405 | - |
Purification (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-arabinose | very poor substrate | Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-glucosamine | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-mannose | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-xylose | - |
Acetivibrio thermocellus | ? | - |
? | |
cellobiose + phosphate | - |
Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 85000, gel filtration, SDS-PAGE | Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | very low activity at 20 and 80°C | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
unstable above | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | - |
Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | most stable at pH 7, rapid loss of activity at different pH | Acetivibrio thermocellus |