Protein Variants | Comment | Organism |
---|---|---|
A423S | mutation introduced to increase thermostability, mutant displays a half-life of 11.3 min at 70°C | Acetivibrio thermocellus |
A781K | mutation introduced to increase thermostability, mutant displays a half-life of 15.3 min at 70°C | Acetivibrio thermocellus |
Q130H/K131Y/S411G/A423S/A781K | mutations introduced to increase thermostability, mutant displays a half-life of 17.7 min at 70°C | Acetivibrio thermocellus |
R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K | mutations introduced to increase thermostability, mutant displays a half-life of 24.6 min at 70°C | Acetivibrio thermocellus |
S411G | mutation introduced to increase thermostability, mutant displays a half-life of 13.6 min at 70°C | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
cellobiose | mutant S411G, pH 7.2, 40°C | Acetivibrio thermocellus | |
10.3 | - |
cellobiose | mutant A423S, pH 7.2, 40°C | Acetivibrio thermocellus | |
11 | - |
cellobiose | mutant A781 K, pH 7.2, 40°C | Acetivibrio thermocellus | |
14.7 | - |
cellobiose | mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
15.9 | - |
cellobiose | mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
16.5 | - |
cellobiose | wild-type, pH 7.2, 40°C | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | - |
Acetivibrio thermocellus | D-glucose 1-phosphate + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cbp | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type | Acetivibrio thermocellus |
80 | - |
mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life of wild-type 8.3 min, mutant Q130H/K131Y/S411G/A423S/A781K 17.7 min, mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K 24.6 min | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
cellobiose | mutant S411G, pH 7.2, 40°C | Acetivibrio thermocellus | |
1.5 | - |
cellobiose | mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
2.1 | - |
cellobiose | mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
2.9 | - |
cellobiose | mutant A423S, pH 7.2, 40°C | Acetivibrio thermocellus | |
2.9 | - |
cellobiose | mutant A781 K, pH 7.2, 40°C | Acetivibrio thermocellus | |
3.4 | - |
cellobiose | wild-type, pH 7.2, 40°C | Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
cellobiose | mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
0.134 | - |
cellobiose | mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C | Acetivibrio thermocellus | |
0.189 | - |
cellobiose | mutant S411G, pH 7.2, 40°C | Acetivibrio thermocellus | |
0.2 | - |
cellobiose | wild-type, pH 7.2, 40°C | Acetivibrio thermocellus | |
0.263 | - |
cellobiose | mutant A781 K, pH 7.2, 40°C | Acetivibrio thermocellus | |
0.287 | - |
cellobiose | mutant A423S, pH 7.2, 40°C | Acetivibrio thermocellus |