EC Number |
Reference |
---|
1.1.1.202 | all genes of the dha regulon, including the gene for 1,3-propanediol oxidoreductase of Klebsiella pneumoniae mobilized by the plasmid RP4:mini Mu and transferred to Escherichia coli |
389493 |
1.1.1.202 | overexpression in Escherichia coli |
389494 |
1.1.1.202 | expression in Escherichia coli |
389495, 710747, 721395, 725951 |
1.1.1.202 | gene TM0920, expression of soluble N-terminally MGSDKIHHHHHH-tagged selenomethionine-labeled enzyme in Escherichia coli methionine auxotrophic strain DL41 |
657320 |
1.1.1.202 | expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli |
667270 |
1.1.1.202 | gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) |
670216 |
1.1.1.202 | gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview |
675901 |
1.1.1.202 | gene dhaT, expression of the N-terminally His-tagged in Escherichia coli strain BL21(DE3) |
684171 |
1.1.1.202 | gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons |
688171 |
1.1.1.202 | overexpression of gene dhaT encoding PDOR (from vector pETPkan-dhaT) and transformed into Klebsiella pneumoniae |
695716 |